纳米银杏对动脉粥样硬化早期大鼠主动脉壁ICAM-1基因表达的影响

The effects of the compounds of Ginkgo Flavone Phospholipid on the expression of intercellular adhension molecule-1 in rat aorta at the early stage of atherosclerosis

目的:观察纳米银杏(银杏黄酮-磷脂复合物)对动脉粥样硬化(AS)早期大鼠主动脉壁细胞间黏附分子-1(ICAM-1)mRNA及其蛋白表达水平的影响。方法:采用生化分析、免疫组化、实时定量荧光RT-PCR以及凝胶电泳,观察纳米银杏对大鼠主动脉ICAM-1mRNA及其蛋白表达水平的影响。结果:与模型组比较,纳米银杏组ICAM-1表达明显降低(P<0.01);与银杏黄酮组比较,纳米银杏组ICAM-1表达明显降低(P<0.01)。结论:银杏黄酮及纳米银杏可下调AS早期大鼠主动脉壁ICAM-1的高表达,纳米银杏的作用明显优于银杏黄酮。

Objective： To observe the effects of the compounds of Ginkgo Flavone Phospholipid （FGP） on the expression of intercellular adhension molecule-1 （ICAM-1）mRNA and protein in rats aorta at the early stage of atherosclerosis （AS）. Methods： The effects of FGP on the expression of ICAM-1 mRNA and protein were observed by biochemistry analysis, immunohistochemistry, RT-PCR and gel electrophoresis. Results： The expression of ICAM-1 of rat aorta of FGP group was obviously lower than the model group （P〈0.01）, and it was also lower compared with the Ginkgo Flavone （GP） group （P〈 0.01）. Conclusion： FG and FGP can downregulate the high expression of ICAM-1 of rat aorta in AS rats. The effect of FGP is superior to FG.