摘要
目的对时间分辨荧光免疫分析技术(TRFIA)检测乙型肝炎病毒(HBV)进行临床应用分析。方法用时间分辨荧光免疫分析技术TRFIA和放射免疫分析(RIA)同时检测261例乙型肝炎病毒感染者和150例正常人血清的HBV标志物,并对两种方法的检测结果HBsAg、HBsAb、HbeAg、HbeAb、HBcAb进行比较分析。同时对TRFIA法HBsg阳性而RIA阴性及TRFIA法HBcAb阴性而RIA阳性用化学发光免疫分析法(ECLIA)进行确诊检验。结果TRFIA法同RIA法比较,检测结果示二者之间差异无统计学意义(P>0.05)。结论TRFIA技术检测HBV标志物较RIA法的敏感度高,特异性强,可作为HBV感染的常规检测方法。
Objective To estim ate clinical application of time-resolved fluorescemce immunoassay (TRFIA) in detecting HBV markers in serum. Method HBsAg, HBSAb, HBeAg, HBeAg, HBCAb in serum in 261patients with hepatitis B virus infection and 150 healthy persons were detected by TRFIA microparticle ewzyme immunoassay (RIA) simultaneollsly. Results The method of TFRIA was compared with RIA, All of them showed there was a powerful conformability between the 2 results of the two menthod, through Х^2 test the results showed that between there was no significant difference (P〉0.05).Conclusion To detect HBsAg markers in serum, TRFIA offers many denefits in sensitivity, specificity, accuracy and practicability, and may de routine instead of RIA and ECLIA.
出处
《中国医药指南》
2009年第12期43-45,共3页
Guide of China Medicine