川芎嗪对百草枯中毒大鼠急性肺损伤的治疗作用

Therapeutical effect of ligustrazine to acute lung injury induced by paraquat in rats

目的探讨川芎嗪对急性百草枯(PQ)中毒大鼠所致肺损伤的治疗作用。方法采用腹腔注射20%PQ溶液制作大鼠急性PQ中毒模型,将大鼠随机分成空白组、阴性对照组、阳性对照组、川芎嗪低剂量组和川芎嗪高剂量组,观察大体标本、组织病理以及肺湿/干质量比、肺泡灌洗液(BALF)中性粒细胞比和蛋白含量。同时测定血清和BALF中MDA含量、SOD和GSH-Px活力以及肺组织核因子-кB(NFк-B)和iNOS活性。结果与阴性对照组相比,川芎嗪低剂量组肺组织淤血、水肿明显减轻,血浆和BALF中MDA含量明显下降(P均<0.01);GSH-Px和SOD活力明显升高(P<0.01)。NF-кB和iNOS降低(P<0.05,或<0.01)。结论川芎嗪可使急性PQ中毒引起的脂质过氧化损害得到改善,且能降低NFк-B及iNOS水平,减轻PQ中毒大鼠肺组织损伤。

Objective To study the therapeutic effect of ligustrazine against acute lung injury by paraquat （PQ）. Methods 50 SD rats were randomly divided into five experimental groups：blank group, negative control group, positive control group, ligustrazine low-dose group（LDG） and figustrazine high-dose group（HDG）. Macroscopic and histopathological examinations were performed and biological indexes were measured for the lung specimens. The indexes included lung wet weight/dry weight, the rats of nentrophils and protein content in the pulmonary alveolar lavage fluid. In the mean time, the levels of MDA, superoxide dismutase（SOD）, glutathione peroxidase（GSH-Px） both in plasma and bronchoalveolar lavage fluid（BALF） were detected. The activities of NK-κB and iNOS in lung tissue homogenate were measured. Results Compared with negative control group, lung congestion and lung edema of ligustrazine low-dose group were mitigated. The level of MDA decreased in both plasma and BALF（ P 〈0.01 ）, and the SOD and GSH-Px activities were increased （ P 〈 0.01 ）. The activities of NK-κB and iNOS in lung tissue homogenate were significantly decreased （ P 〈0.05, 〈0.01 ）. Conclusions Administration of ligustrazine could improve the lipid peroxidation damage and ameliorate the lung injury and decrease the concentrations of NK-KB, iNOS caused by acute poisoning of PQ.