不同给药方式下恩诺沙星在鲤体内的药动学研究

Pharmacokinetic of Enrofloxacin in Carp Following Different Ways of Administration

对两组鲤分别进行腹腔注射、口灌恩诺沙星后,应用高效液相色谱法测定了其组织中的药物浓度,研究了恩诺沙星在鲤(Cyprinus carpio)体内的吸收、分布与消除等药代动力学参数.结果表明:两种给药方式下,鲤血浆、肝脏、肾脏和肌肉组织的药时曲线符合一级消除二室模型.腹腔注射给药血浆动力学参数:AUC为59.1856μg.h.mL-1、Ka为75.7627 h-1、t1/2β为96.5456 h、T(peak)为0.0730 h、C(max)为3.2970μg.mL-1;灌服给药血浆动力学参数:AUC为600.2961μg.h.mL-1、Ka为0.1693 h-1、t1/2β为168.2871 h、T(peak)为3.6655 h、C(max)为3.2661μg.mL-1.这说明腹腔注射给药比口灌给药吸收快,血药达峰时间短,达峰浓度高.

The concentration of enrofloxacin in tissues of carp following enterocoelia injection administration and oral administration was evaluated by HPLC respectively. Several pharmacokinetics parameters such as absorption, distribution and elimination were set up. The results showed that the concentration of enrofloxacin in plasma , liver, kidney and muscle of carp following two different forms of administration was described as a two-compartment model with 1 st order absorption. The pharmacokinetic parameters in plasma by enterocoelia injection administration were： AUC 59. 185 6 μg·h·mL^-1, Ka 75. 762 7 h^-1, t1/2β 96. 545 6 h, T（peak） 0. 073 0 h, and C（max） 3. 297 0 μg·mL^-1; the pharmacokinetic parameters in plasma after oral administration were AUC 600. 296 1 μg·h·mL^-1, Ka 0. 169 3 h^-1, t1/2β 168. 287 1 h, T（peak） 3.665 5 h, and C（max） 3. 266 1 μg·mL^-1. These results indicated that enterocoelia injection administration led to more rapid absorption, less retaining time and higher peak concentration than oral administration.