血红素加氧酶-1基因多态性,神经元特异性烯醇化酶与脑梗死关系的研究

The relationship between heme oxygenase-1 gene promoter polymorphism,nse and cerebralinfarction

目的探讨血红素加氧酶-1基因启动子区域GT重复序列[HO-1(GT)n]遗传多态性,神经元特异性烯醇化酶(NSE)与脑梗死的遗传易感性的关系。方法采用巢式PCR法检测HO-1(GT)n,酶联免疫吸附法(ELISA)检测血清NSE,分析55例急性脑梗死(ACI组)患者和56例对照者的外周血样本。结果ACI组短GT重复序列分布43.64%明显低于对照组64.29%,差别有统计学意义。在急性脑梗死患者治疗前后均显示短GT重复序列组NSE水平低于长GT重复序列组NSE水平。结论HO-1基因启动子短重复序列脑梗死患者血清NSE水平较低,HO-1短GT重复序列遗传多态性可能与脑梗死的遗传易感性有关。

Objective To investigate the interrelation between genetic polymorphism of Heme oxygenase-1 genic repeated sequence in promoter region（ HO-1 （GT）n）and serum levels of neuron-specific enolase（NSE） in hereditary susceptibility of cerebral infarction. Methods The information and peripheral blood sample came from 55 acute cerebral infarction （ACI group）and 56 healthy people as control group were collected. HO-1 （GT）n was detected by PCR, and NSE was detected by ELISA. Results The number of HO-1 genotype with S allele was significantly different between the ACI group （43.64%） and control group（65.45% ）. The level of serum NSE before treatment in ACI-group was significantly different with that of after treatment ACI-group and control group（P 〈 0.05 ）. Conclusion The ACI patients whose HO-1 genotype with S allele have lower level of serum NSE than the ACI patients whose HO-1 genotype without S allele, and neuron was less damaged. The less repetitive sequence exhibits a reduced risk for ACI.