Runx3基因启动子甲基化及其蛋白表达与喉癌的关系

Relationship of Methylation of the Runx3 Gene and Expression of Protein in Laryngeal Carcinoma

目的探讨Runx3基因启动子甲基化状态及其蛋白表达与喉癌临床病理特征的关系。方法采用免疫组化SP法检测55例喉癌组织及癌旁正常黏膜组织中Runx3DNA的表达,并用甲基化特异性PCR(MSP)方法检测Runx3基因启动子甲基化情况。结果55例喉癌组织标本中Runx3基因蛋白的表达较癌旁正常组织中表达明显降低,差异有统计学意义(P<0.05);癌旁正常黏膜组织中无Runx3基因启动子的甲基化,喉癌组织中有32例(58.2%)检测到Runx3基因启动子的甲基化,2者比较差异有统计学意义(P<0.05);喉癌组织中Runx3基因启动子甲基化及Runx3蛋白的表达与性别、临床分型及分级、有无淋巴结转移均无关(均P>0.05);喉癌组织中Runx3基因启动子甲基化与Runx3蛋白表达呈负相关(r=-0.7092,P<0.01)。结论Runx3基因启动子甲基化是其基因失活的可能机制之一,在喉癌的发生中起重要作用。

Objective To investigate the relationship between methylation and the expression of Runx3 in promoter region and and its possible relationship with the clinicopathological features of human laryngeal carcinoma. Methods Using immunohistochemistry technique,specimens from 55 patients of carcinoma of larynx （tumor tissues,adjacent normal tissues） were detected for their expression of the Runx3 gene. Meanwhile,Methylation-specific PCR was used to detect methylation of Runx3 at promoter region. Results The expression of Runx3 protein in carcinoma of Larynx was down-regulated than that in adjacent normal tissues samples（P〈0.05）. No methylation of Runx3 promoter was found in adjacent normal tissues samples, but was detected in 32 cases （58.2%）of 55 carcinoma of larynx. The rate of methylation of Runx3 promoter in carcinoma of larynx was higher than that of in adjacent normal tissues（P〈0.05） ,and it was not related to the gender.clinical classification, tumor pathological grade and lymph node metastasis （P〈0. 05）. There was correlation between methylation of Runx3 gene promoter and expression of its protein （r= - 0. 709 2,P〈0.01）. Conclusion Hypermethylation may cause the Runx3 gene inactivation in human carcinoma of Larynx. Methylation of runx3 promoter maybe correlated with genesis of carcinoma of larynx.