金针菇子实体多糖分离最佳工艺研究

Studies on Optimal Technical Conditions of Polysaccharides Isolation from Flammulina Velutipes

对金针菇子实体多糖提取、纯化条件进行优化研究，结果表明：金针菇多糖提取最佳工艺条件是加水量为样品３０倍，８０℃浸提１ｈ，醇析乙醇终浓度为６０％～７０％，蛋白质去除为氯仿／正丁醇（Ｖ／Ｖ），氯仿＋正丁醇／样品（Ｖ／Ｖ）分别为１∶０．２和１∶０．２４时，效果最好。

Isolation of polysaccharide from flammulina velutipes has been studied. The optimal conditions of extracting polysaccharide from mushroom as following: adding water volume 30 times as much as that of sample,extracting lh at 80℃. The terminal concentration of alcohol for precipitating is about 60%￣70%. The best results of protein removal with the volume ratio of chloroform to butyl alchol and that of sum volume of sample to the ratio of same are 1∶0.2 and 1∶0.24 respectively.