半胱氨酸合成酶基因CPCSaseA植物表达载体的构建

Construction of the Plant Expression Vector of the Cysteine synthase gene CPCSaseA

半胱氨酸合成酶是植物硫代谢过程中催化O-乙酰丝氨酸和硫化氢合成半胱氨酸的关键酶。用全长cDNA文库EST随机测序的方法获得了蜡梅的半胱氨酸合成酶胞质异形体基因,构建了全长正义植物表达载体pB I121-CPOASTL。

Cysteine synthase （CSase） , a key enzyme of plant sulfur metabolism, catalyzes the formation of Cys from sulfide and O - acetylserine. In this paper, the gene of cytosolic isoform of Cysteine synthase was isolated from Chimonanthus praecox, adopting the method of randomly sequencing the ESTs of full - length cDNA library, Constructed a full - length and sense plant expression vector pBI121 - CPOASTL, Laid a solid foundation for further work.