黑莓种子发芽试验

Experiment on the Seed Germination of Blackberry

[目的]为黑莓的栽培及育种筛选出较好的发芽方法。[方法]以黑莓栽培品种成熟鲜果中的饱满种子为材料,种子设3个不同处理:A、在胚根端剥离内果皮,保留种皮及胚乳层;B、在胚乳端剥离内果皮,保留种皮及胚乳层;C、不剥离。然后,用3种培养方法对黑莓种子进行发芽试验。[结果]在双层湿滤纸光培养中,处理A和B的种子发芽率均在40%左右,处理C的种子没有发芽;在双层湿滤纸暗培养中,处理A和B的种子发芽率均在61%以上,最高达63.6%,处理C的种子没有发芽。基质培养的种子的最高发芽率为15.1%。不同处理种子生成的小芽苗的移栽成活率均为100%。在培养基培养中,激素浓度配比对黑莓种子的发芽率没有影响。[结论]双层湿滤纸暗培养法是黑莓种子的理想发芽方法。

[ Objective ] The purpose was to screen out a better germination method for cultivating and breeding blackberry. [ Method ] With the full seeds from ripe and fresh fruits of blackberry cultivars as materials, they were treated by 3 different methods ： A, the endocarp on radiculodium was peeled and the seed coat and endosperm layer were remained; B, the endocarp on endosperm end was peeled and the seed coat and endosperm layer were remained; C, non-peeled. Then, the germination enperiment was performed on the blackberry seeds by 3 culture methods. [ Result] In the light culture with double-layer wet filter paper, the seed germination rates in both Treatment A and B were about 40% and there was no seed germinated in Treatment C. In the dark culture with double-layer wet filter paper, the seed germination rates in both Treatment A and B were higher than 61%, their highest germination rate was up to 63.6% and there was no seed germinated in Treatment C. The highest germination rate of seeds cultured on medium was 15.1%. The transplanting survival rates of germule seedlings grown from seeds treated by different methods were 100%. In the culture on medium, the hormone concentration combination had no effect on the seed germination rate of blackberry. [ Conclusion ] The dark culture with double-layer wet filter paper was an ideal germination method for blackberry seeds.