奶牛布鲁氏杆菌的分离与鉴定

Isolation and Identification of Dairy Cattle Brucellosis

[目的]为布鲁氏杆菌的分离与鉴定提供依据。[方法]时某奶牛场进行布病调查，采集血清、血液、牛奶等样品通过硫化氢产，生试验、染料抑菌试验和热凝集试验进行布鲁氏抒菌的分离与鏊定。[结果]21份牛奶样品在浓度10％CO2培养葙中培养至第4天时，有5份培养基上生长出30～50个0．05～2．00mm大小无色、闪光圆形、湿润、隆起的可疑小菌落。硫化氢产生试验结果表明，5个菌株均可产生硫化氢；染料抑菌试验结果表明，5个菌株确为布鲁氏杆菌；热凝集试验结果表明，该5菌株未发生变异；PCR检测结果表明，相对应的牛奶检测呈阳性。[结论]初步鉴定从布病可疑阳性牛分离出的5个菌株为牛布鲁氏杆菌。

[ Objective ] The basis of the isolation and identification of dairy cattle brucellosis was provided. [ Method ] The investigation of the cattle disease caused by brucellosis was conducted. A dairy farm survey was made, After collection of serum, blood, milk and other sampies the hydrogen sulfide test, test, and thermal dye inhibition agglutination test were conducted for brucellosis isolation and identification. [ Results] The 21 milk samples in 10% CO2 concentration in the culture incubator to 4 days, 5 growth medium of 30- 50, 0.05 -2.00 mm Size colorless, flashing round, moist, small colony of the suspicious bulge. The results showed that hydrogen sulfide produced, five strains may have a hydrogen sulfide ; dye bacteriostasis test results show that indeed for the five strains of brucellosis; heat agglutination test results show that the 5 strains did not mutate; PCR test results show that the , corresponding to the milk tested positive. [ Conclusion ] cloth from the initial identification of disease-positive cattle suspected isolated five strains for bovine brucellosis.