摘要
目的研究花青素对四氯化碳(CCl4)所致的急性化学性肝损伤的预防作用。方法昆明种小鼠60只,雌雄各半,随机分为空白对照组、肝损伤模型组、阳性对照组(给予维生素E)及花青素低、中、高剂量组,用分光光度法测定并比较各组小鼠肝脏指数,脾脏指数及胸腺指数,红细胞膜中超氧化物歧化酶(SOD)、过氧化氢酶(CAT)的活力和丙二醛(MDA)的含量,血浆中谷胱甘肽过氧化物酶(GSH-Px)、丙氨酸氨基转移酶(ALT)、天冬氨酸氨基转移酶(AST)活力以及肝糖原水平。结果花青素可抑制由于氧化损伤所致的小鼠红细胞膜中SOD、CAT活性的降低,抑制血浆中GSH-Px活性的降低,降低血浆ALT、AST、红细胞膜中MDA及肝脏指数的异常升高;提高肝糖原水平及脾脏指数及胸腺指数。结论花青素对四氯化碳所致氧化损伤的小鼠有明显的保护作用。
Objective To study the protective effects of anthocyanidin against the acute liver injury induce by CCl4 in mice. Methods 60 Kunming mice with equal numbers of male and female were randomly divided into control group, liver injury model group, VE group and groups with low, middle and high dose of anthocyanidin. Liver index, spleen index, thymus index, activities of erythrocyte membrane superoxide dismutase （SOD）, plasma alanine transaminase （ALT）, aspartate transominase （ AST）, glutathione perioxidase （GSH-Px） and catalase（CAT） , malondialdehyde （MDA） , and hepatic glycogen were assayed and compared between groups. Results Anthocyanidin significantly increased activities of SOD, CAT and GSH- Px; increased hepatic glycogen, spleen index, and thymus index;abated plasma ALT,AST, MDA content and liver index. Conclusion Anthocyanidin shows protective effects on the acute liver injury induced by CCl4 in mice.
出处
《医药导报》
CAS
2009年第7期843-846,共4页
Herald of Medicine
关键词
花青素
抗氧化
损伤
肝
急性
Anthocyanidin
Antioxidation
Injury ,liver , acute
