仿刺参体腔细胞吞噬与凝集功能及其与温度的关系

Phagocytosis and agglutination of coelomocytes in sea cucumber Apostichopus japonicus with relation to water temperature

采用光镜和电镜技术研究了仿刺参Apostichopus japonicus体腔细胞的吞噬和凝集功能及其与温度的关系。结果表明:仿刺参体腔细胞中球形细胞和变形细胞具有较强的吞噬功能,当异物的大小、性质不同时,其吞噬过程和吞噬能力存在一定差异。当体腔细胞吞噬洋红颗粒时,试验进行6 h其吞噬率最高(48.01%),108 h时吞噬过程结束;当体腔细胞吞噬酵母时,试验进行4 h其吞噬率最高(35.17%),且体腔细胞的凝集现象较多,到96 h时吞噬过程结束。仿刺参体腔细胞的吞噬能力与温度关系密切,18℃下吞噬百分率和吞噬指数最高,12℃下次之,4℃和25℃下最低。仿刺参离体的体腔细胞很快发生凝集反应,且温度和异物刺激都会影响体腔细胞的凝集作用时间和强度。

Phagocytosis and agglutination of coelomocytes in sea cucumber Apostichopus japonicus with the relation with water temperature were studied by light and electron microscopes. The phagocytosic assay showed that Spherolocytes and Amoebocytes had the abilities to phagocytize garmine and yeast, and the abilities of phagocytosis were correlated to the kind and size of the foreign bodies. In the assay of phagocytizing garmize, the maximal phag- ocytosis （48.01%） was observed 6 hours later, and 108 hours later the phagocytosis was over. In the assay of phagocytizing yeast, the maximal phagocytosis （35.17%） was occurred at 4 hours later, and the phagocytosis was over 96 hours later. Furthermore, the type III Spherolocyted only phagocytize garmine. In addition, the function of phagocytosis was correlated to temperature. The peak phagocytic percent and index appeared at 18℃, which was higher at 12℃ than that at 4℃ and 25℃. The coelomocytes had the abilities to agglutinate in vitro and the agglutination was correlated to the stimulation by temperature and foreign bodies.