亚硝酸盐氧化法测定血清总胆红素的技术性能评价

Technical property evaluation of the enitrate oxidation method for assay of serum bilirubin

目的 观察亚硝酸盐氧化法用于血清总胆红素测定的价值。方法分别采用亚硝酸盐氧化法和重氮试剂法等测定血清总胆红素水平，并对其技术性能进行分析。结果亚硝酸盐氧化法测定结果与重氮试剂法和钒酸盐氧化法均有良好的相关性，总胆红素日内CV为0．81％-2．59％、日间CV为1．33％，平均回收率为100．6％，线性浓度达342／μmol／L；溶血对总胆红素测定无显著干扰，对直接胆红素仅有轻度负干扰，脂血对测定无明显干扰。结论亚硝酸盐氧化法测定血清总胆红素水平精密度、准确度高，稳定性、抗干扰能力强，且其试剂价格低廉、使用方便，值得推广应用。

Objective To investigate the value of enitrate oxidation method for measuring serum total bilirubin （TBIL）. Methods The serum TBIL in 566 eases was determined by enitrate oxidation method and diazo method respectively,and the technical property of the former was evaluated. Results There was a good con＇elation between the enitrate oxidation method and diazo method, vanadilcm-aeid oxidalion method. The daily and day CV of TBIL were 0.81%- 2.59% and 1.33% respectively, the average recovery rate was 100.6% respectively, the linear range was 342.0 μmol/L. Hemolysis showed no significant interference on the determination of TBIL, hut a little negative interference on the determination of direct BIL , and lipidemia showed no significant interference on the determination. Conclusions The enitrate oxidation method for assay of serum bilirubin has the features of high precision and accuracy, strong stability and anti-jamming ability,low cost of reagent and convenience of use,and valuable to be popularized.