楸树组培初代培养技术

Technique of Inducing in Vitro Culture of Catalpa bungei C.A. Mey

以楸树幼嫩茎段为试验材料,对组培初代培养阶段外植体无菌体系的建立和腋芽诱导影响因素进行了研究探讨。结果表明:茎段外植体最佳灭菌措施为:自来水冲洗30 min→70%酒精浸泡6 s→无菌水冲洗2次→(100mg/L青霉素+100 mg/L链霉素)浸20 min→0.1%升汞液浸泡10 min→无菌水冲洗8次→100 mg/L PVP浸泡5min。楸树茎段腋芽诱导的最适宜培养基为:1/2MS+BA1.0 mg/L+ZT0.2 mg/L+IBA0.2 mg/L。添加食糖30 g/L琼脂8g/L,调整pH5.8。

A Study was conducted on building explant asepsis system in primary culture of Catalpa bungei and influencing factor about the axillary bud induction with C. bungei stem segments explant . The result showed that the effective procedure of setting up asepsis shoots was the following ： washed 30 min by tap water→dipped in 70% alcohol →washed 2 times by aseptic water→ dipped in the 100 mg/L penicillin and the 100 mg,/L streptomycin about 20 min→ dipped in the 0. 1% Hgcl2 10 min→washed 8 times by aseptic water→ soaked in the 100 mg/L PVP 5min→ inoculated . The best induction culture medium of the axillary buds of C. bungei was 1/2MS ＋ BA1.0 mg/L ＋ ZT 0. 2 mg/L ＋ IBA0.2 mg/L ＋ sugar 30 g/L ＋ agar 8 g/L, pH5. 8.