GPAT/AIRC基因对白耳鸡群体肌肉肌苷酸含量的遗传效应分析

Analysis of the Genetic Effects of GPAT/AIRC Genes on IMP Content in Baier Chicken

以GPAT/AIRC基因为侯选基因,以白耳鸡为试验材料,采用PCR-SSCP方法对GPAT基因外显子2,AIRC基因外显子8进行SNPs检测。结果各发现了一个单核苷酸多态性位点。各种基因型与胸肌IMP含量的方差分析结果表明:GPAT基因外显子2中AA型个体的肌肉肌苷酸含量显著地高于AB型和BB型个体,AB型个体也稍高于BB型,但差异不显著;AIRC基因外显子8中CG型个体的肌肉肌苷酸含量显著地高于GG型和CC型个体,GG型个体的胸肌肌苷酸含量也显著地高于CC型。两个基因的单倍型对IMP含量也有显著的影响:单倍型为AACG的个体比单倍型为BBCC的个体高1.006 mg/g,单倍型的遗传效应大于最优秀的单个基因型效应。因此,可以利用该单倍型对鸡的肉质风味性状进行标记辅助选择。

This study was designed to investigate the effect of GPAT/AIRC gene on muscle IMP content in Baier chickens. The primers for the exon2 in GPAT gene and exon8 in AIRC gene were designed and the SNPs were detected by PCR-SSCP and DNA sequencing. One SNP were detected in exon2 of GPAT gene and exon8 of AIRC gene, respectively. The least square analysis showed that AA genotype birds had significant higher IMP content than AB and BB genotype birds, AB genotype birds also had a little higher IMP content than BB genotype birds, but the difference was not significant in GPAT gene exon 2. In AIRC gene exon 8, CG was the excellent genotype, individuals with CG genotype had significantly higher IMP content than GG and CC genotype birds, GG genotype birds also had significantly higher IMP content than CC genotype birds. The haplotypes of GPAT gene exon 2 and AIRC gene exon 8 also had significant genetic effect on IMP content. The IMP Content of the individu- als with AACG haplotype was 1. 006 mg/g higher than the individuals with BBCC haplotype. The genetic effect of the haplotype was larger than the excellent single genotype effect. Therefore we putatively drew the conclusion that the haplotypes could be used as the molecular genetic marker to select the chicken for meat quality trait.