摘要
目的:研究白藜芦醇(Resveratrol)作用前后CD34+CD38-KG1a白血病细胞对IL-15介导的人外周血单个核细胞(PBMC)杀伤敏感性的变化及机制的初步探讨。方法:应用CCK-8法检测白藜芦醇对白血病细胞的IC50值,以此量的白藜芦醇作用于白血病细胞.,并用LDH释放法检测白藜芦醇作用前后,效靶比分别为5∶1、10∶1、20∶1的IL-15介导的PBMC对CD34+CD38-KG1a细胞杀伤能力。流式细胞仪(FACS)检测IL-15介导前后PBMC表面NKG2D的表达。流式细胞仪(FACS)检测作用前后CD34+CD38-KG1a细胞表面NKG2D配体(MICA、MICB、ULBP1、ULBP2、ULBP3)表达。结果:白藜芦醇作用前后在不同效靶比时对IL-15介导的PBMC杀伤敏感性差异有统计学意义(P<0.05)。IL-15介导PBMC前后表面NKG2D的表达有显著变化,差异有统计学意义。白藜芦醇作用前后CD34+CD38-KG1a细胞表面NKG2D各配体中MICA、MICB无显著变化(P>0.05),ULBP1、ULBP2、ULBP3有显著变化,差异有统计学意义(P<0.05)。结论:白藜芦醇可以提高CD34+CD38-KG1a细胞对IL-15介导PBMC的杀伤敏感性,其机制可能与白藜芦醇提高CD34+CD38-KG1a细胞表面NKG2D配体的表达有关。
Objective： To explore the effects of PBMC stimulated by IL-15 on leukemic cell lines CD34^＋ CD38^- KGla treated by Resveratrol.Methods：IC50 of CD34^＋ CD38^- KGIa treated by Resveratrol for 48 h was measured by CCK-8 assay and CD34^＋ CD38^- KGla cells were treated with the level of Resveratrol .PBMCs stimulated by IL-15 against CD34^＋ CD38^- KGla cells were analyzed by LDH releasing assay before and after treated by Resveratrol. Protein expression of NKG2D of PBMC stimulated by IL-15 was assayed by flow cytometery. Protein expressions of NKG2D ligands and HLA-class I molecules were assaied by flow cytometery before and after treated by Resveratrol. Results： PBMCs against CD34^＋ CD38^-KGla cell treated by Resveratrol was enhanced at different effector-to-target（ P 〈 0.05）.There was significant difference of the expression of NKG2D on PBMCs stimulated by IL- 15 （ P 〈 0. 05）. The expression of NKG2D ligands of CD34^＋ CD38^- KGla cell were up-expressed after treated by R esveratrol （ P 〈 0.05）. There was no significant difference of expression HLA-class I molecules between the groups before and after treated by Resveratrol （ P 〉 0.05）. Conclusion： The Resveratrol could obviously up-fegulate the NKG2D ligands expression of CD34^＋ CD38^- KGla cells and enhance the cytotoxicity of PBMC against them.
出处
《中国免疫学杂志》
CAS
CSCD
北大核心
2009年第7期622-625,共4页
Chinese Journal of Immunology
基金
广东省中医药局科研课题(2007180)
