反式白藜芦醇分子印迹复合膜的制备及其选择性

Preparation of Resveratrol Molecularly Imprinted Composite Membranes and Its Selectivity to the Template

以反式白藜芦醇为模板分子,聚偏氟乙烯微孔滤膜为支撑膜,丙烯酰胺为功能单体,乙二醇二甲基丙烯酸脂(EDMA)为交联剂,采用热引发原位聚合方法制备了白藜芦醇分子印迹聚合物膜。研究了分子印迹膜对白藜芦醇及其结构类似物(2-萘酚、白藜芦醇甙和双酚A)的结合和透过性,并用扫描电子显微镜对膜的形貌进行了观察。结果表明,印迹复合膜对模板分子白藜芦醇的吸附量远远大于其它结构类似物,其饱和吸附量达1.72μmol/g,为非印迹膜的3倍;尺寸比模板分子小的2-萘酚最先透过,而相对于尺寸接近或大于模板分子的双酚A或白藜芦醇甙,则模板分子优先透过,而且模板分子在印迹膜上的透过量大于非印迹膜。

Resveratrol （RES） imprinted composite membranes, with polyvinylidene fluoride microporous membrane as supporting membrane, were synthesized via in situ polymerization by thermal initiation, using acrylamide（AM） as the functional monomer and ethyleneglycol dimethacrylate（EDMA） as the cross-linking agent. Scanning electron microscope （SEM） was used to visualize the surface of membranes. The binding ability and permeation selectivity were studied for RES and its analogues, polydatin（ POL）, bisphel A（BPA） and 2-naphthol. The results indicate that the imprinted membranes demonstrate satisfactory recognition capacity and selectivity towards the template molecules. Rebinding of RES was much higher than those of structurally similar compounds. The saturated binding capacity of the imprinted composite membrane for RES is about 1.72 μmol/g, nearly 3 times that of non-imprinted polymer membrane. 2-Naphthol, which is smaller than the template molecule in size, permeates through the imprinted composite membrane preferentially. The permeation amounts of BPA and POL are both less than that of RES whether the molecular size is the same as or bigger than RES. RES permeates quickly through the imprinted membrane probably due to its complementary functional groups and shaping with the imprinted sites on the imprinted composite membrane. The RES transfer rate is increased through the imprinted composite membranes probably due to the larger amount of binding sites, compared with that through the non-imprinted one.