钴胺素溶析结晶过程及离子杂质对其晶体生长的影响

Crystallization of Cobalamineand the Influence of Ionic Impurities on Its Crystal Growth

采用激光法研究了钴胺素的结晶过程热力学,考察了温度、溶剂对溶解度和超溶解度的影响,并测定了介稳区,介稳区的宽度随体系中丙酮含量的减小而变宽。研究了离子杂质Na+、Mg2+、Ca2+和Fe3+对钴胺素结晶诱导期的影响。结果表明,离子的存在使得晶体更容易出现断裂和聚结,并用扫描电子显微镜测试技术对晶体的表面形貌进行了表征。结果表明,Fe3+、Mg2+比Na+、Ca2+影响更明显。

This paper presents an experimental investigation on the crystallization thermodynamics of cobalamine by means of laser. The influences of temperature and different solvents on the solubility and supersolubility are studied. The metastable zone is also measured. It shows that the metastable zone width becomes wider with decreasing the proportion of acetone. The influences of four typical ionic impurities （ Na ^＋ , Mg^2＋ , Ca^2 ＋ and Fe^3＋ ） on cobalamine crystallization are studied. Some of crystallization products become fragmentation and agglomeration with ionic impurities existence. The surface configuration has been characterized by SEM. As a result, it shows that Fe^3 ＋ and Mg^2＋ have more significant effects than Na ^＋ and Ca^2＋.