人舌癌移植瘤模型中血管内皮细胞来源的实验研究

The origin of vascular endothelial cell in nude mice bearing human tongue carcinoma

目的观察人舌癌裸鼠皮下移植瘤模型中肿瘤新生血管内皮细胞的来源。方法通过皮下移植人舌癌Tca8113-M1细胞建立人舌癌裸鼠移植肿瘤模型，并于肿瘤生长至直径1cm时切除肿瘤，对移植瘤进行病理组织学观察，利用免疫组织化学方法检测移植瘤内新生血管内皮细胞的来源，抗体为鼠抗人单克隆抗体CD34和大鼠抗小鼠单克隆抗体CD34；并进行微血管密度（microvessel density，MVD）计数及血管内皮生长因子（vascular endothelial growth factor，VEGF）表达检测。结果右腋窝皮下接种人舌癌Tca8113-M1细胞2周后，6只裸鼠的肿瘤直径都达到1cm以上，切片HE染色可见肿瘤组织病理形态为鳞状上皮细胞癌，MVD平均值为10．72±2．12，其中肿瘤新生血管内皮细胞大鼠抗小鼠CD34免疫组化结果是阳性，而鼠抗人CD34阴性。VEGF在6例移植瘤标本中均阳性表达，平均阳性率为（67±5．6）％。结论人舌癌移植瘤模型中血管内皮细胞主要来源于宿主裸鼠，并且可能与肿瘤细胞分泌的VFGE诱导有关。

Objective To explore the origin of vascu/ar endothelial cells in human tongue carcinoma xeno- graft model by nude mice. Methods The model of human tongue carcinoma xenograft was established by transplan- ting Tca8113 -M1 subcutaneously to nude mice ,to identify the germinal origin of neovascularization and assay the mi- eroangiogenesis, the neoplasms（ 〉 1cm in diameter） induced by Tea8113 -MI were removed and dealt with his- topathologieally through immunohistoehemieal staining of VEGF and CD34（ including rat - anti - human and rat - an- ti - mouse CD34）. Results HE staining showed squamous eell eareinoma; positive expression was observed in rat - anti - mouse CD34 staining and MVD averaged 10. 72 ± 2. 12, meanwhile no visible alteration of rat - anti - human CD34 staining;moreover, VEGF staining presented a 100% positive ration in six transplanted tumor and the numbers of positive cells averaged （67±5.6）% in every slice. Conclusion The germinal origin of vascular endothelial eells in Tea8113 -M1 xenograft model derived from host nude miee, and may be induced by VEGF whieh is secreted by tumor cells.