根霉产纤溶酶液体发酵培养基的优化

Optimization of liquid fermentation medium for fibrinolysin production by Rhizopus chinesis

通过单因素,均匀设计和正交试验,从碳氮比、无机氮源、胰蛋白胨、pH值方面优化了根霉液体发酵产生纤溶酶的培养基组成。试验结果表明,试验范围内菌株液体发酵产纤溶酶的适宜培养基组成为:麸皮水5.6%,豆粕水解液5.3%,二者体积比为2:3,磷酸氢二铵0.4%,硝酸钠0.5%,pH值自然。优化培养基的纤溶酶酶活力为140.00U/mL。

The liquid fermentation medium for fibrinolysin production by Rhizopus chinesis was optimized by single-factor test, uniform design and orthogonal test in terms of carbon/nitrogen ratio, inorganic nitrogen source, tryptone and pH. The results showed that the suitable medium consisted of 5.6% wheat bran solution, 5.3% soybean cake hydrolysate, 0.4% （NH4）2HPO4 and 0.5% NaNO3 with natural pH and volume ratio of wheat bran so- lution to soybean cake hydrolysate 2：3. The fibrinolysin activity reached 140.00 U/ml on optimized medium.