黑曲霉利用菠萝皮培养基发酵产果胶酶的研究

Pectinase production by Aspergillus niger with pineapple peel medium

为了摸索菠萝皮综合利用的新途径,对以菠萝皮为主要碳源和诱导物的黑曲霉果胶酶发酵进行了初步研究。结果表明,在菠萝皮最佳用量的培养基中,硫酸铵为最佳无机氮源,适量添加麸皮、Mg2+、Ca2+以及Tween-20对产酶具有促进作用。本次试验确定培养基主要组分为:菠萝皮粉60g/L,麸皮12.5g/L,(NH4)2SO420g/L,NaH2PO410g/L,MgSO4·7H2O1g/L,CaCl21.1g/L,Tween-203g/L;以此培养基进行发酵,最高果胶酶活力可达76818U/mL。

In order to find the new way for comprehensive utilization, pectinase production by Aspergillus niger with pineapple peel medium was studied primarily. The results indicated that （NH4）2SOawas the best inorganic nitrogen sources, and pectinase production could be improved by adding adequate wheat bran, Mg2~, Ca2＋ and Tween-20.The optimal medium components were as follows： pineapple peel 60 g/L, wheat bran 12.5 g/L, （NH4）2 SO4 20g/L, NaH2PO4 10 g/L, MgSO4.7H2 0.1 g/L, CaCl2 1.1 g/L, Tween 20 3g/L. Under these conditions, the pectinase activitycould reach to 76818 U/mL.