柠檬酸废水厌氧发酵产沼气的研究

Methane production by anaerobic batch fermentation of citrate wastewater

利用厌氧培养系统,在37℃和120r/min分批培养过程中周期性取样,考察柠檬酸废水厌氧发酵产沼气的可行性与优越性。研究发现废水中的柠檬酸促进了挥发性乙酸盐底物向沼气的转化,YP/V为378mg/g,比对照提高18.4%;同时,提高了菌群厌氧发酵产沼气的效率,其YP/T为230mL/g,比对照提高30.7%。此外,还促进了菌群对挥发性乙酸盐底物的利用能力,其qV为7.52mg/g·h,比对照提高10.6%,而且菌群合成沼气的能力为2.93mL/g·h,比对照提高了和35.7%。试验结果证实了柠檬酸废水对产气的促进是通过提高菌群的甲烷合成能力来提高产气效率,而非仅通过促进菌群生长的群体优势,并说明柠檬酸废水厌氧发酵产沼气具有较高的效率和较好的应用前景。

The effects of citrate wastewater on methane production during the anaerobic batch fermentation were investigated, using anaerobic system including filter flask, at 37℃ and speed 120 r/min, sampling periodically. The results showed that the methane fermentation was stimulated; YP/v, YP;r, qv and qp was 378 mg/g, 230 ml/g, 7.52 mg/g-h and 2.93 ml/g .h, which was 18.4%, 30.7%, 10.6% and 35.7% higher than control, respectively. The results suggested that citrate wastewater facilitated biogas production not only by increasing microorganism amounts, but also by enhancing the ability of microorganisms to biosynthesize methane.