油茶苯丙氨酸解氨酶基因的cDNA克隆与序列分析

Molecular clone and sequence analysis of phenylalanine ammonia lyase gene from Camellia oleifera

苯丙氨酸解氨酶(phenylalanine ammonia lyase,PAL)是苯丙烷类代谢途径的关键酶之一。以油茶(Camelliaoleifera)近成熟种子cDNA文库和EST文库为材料,通过分子克隆方法鉴定了PAL全长cDNA。结果表明:油茶PAL基因的cDNA长2 118 bp,编码705个氨基酸,与其他物种的PAL具有较高的相似性,在进化上高度同源;油茶PAL编码的蛋白质序列包含与水稻、玉米PAL蛋白质相同的脱氨基位点和催化活性位点;PAL系统进化树表明,油茶PAL基因与灌木类植物的PAL基因聚类关系最近。

PAL （phenylalanine ammonia lyase） is one of the key enzymes in plant secondary metabolism. The fulllength eDNA of PAL was identified from the eDNA library and EST library of Camellia oleifera by the method of molecular cloning in this paper. The results showed that the cDNA of C. oleifera PAL contained 2 118 bp, encoding 706 amino acids, and had a high similarity in the evolution of a high degree of homology with other species of PAL; C. oleifera PAL encoded protein sequence contained the same deamination site and catalytic active site with protein PAL of rice and corn. PAL phylogenetic tree analyses revealed that the C. oleifera PAL had closer relationship with PALs from Shrub plants than from other plants.