微波辅助预处理对玉米秸秆酶解的影响

Effects of microwave-assisted pretreatment on the enzymatic hydrolysis of corn stover

研究了微波辅助硫酸、氢氧化钠预处理对玉米秸秆酶解糖得率的影响,并对温度、酸碱添加量、预处理时间、液固比4个因素进行了单因素试验分析。结果表明预处理的最佳条件为:微波-硫酸预处理时,温度190℃,硫酸质量浓度为10 g/L,预处理时间3 min,液固比20(硫酸体积(mL)与玉米秸秆质量(g)之比)条件下,预处理得糖率及酶解得糖率分别为44.6%和30.3%;微波-氢氧化钠预处理时,温度130℃,氢氧化钠质量浓度15 g/L,预处理时间7 min,液固比30(氢氧化钠溶液体积(mL)与玉米秸秆质量(g)之比)条件下,预处理得糖率及酶解得糖率分别为1.5%和80.0%。

The effects of microwave-assisted sulfuric acid and sodium hydroxide pretreatment on sugar yields of enzymatic hydrolysis of corn stover were studied. Four factors, i. e. , temperature, acid or alkali concentration, pretreatment time and liquid to solid ratio were selected in the single factor analysis method. It was found that with microwave-assisted sulfuric acid pretreatment the optimal pretreatment conditions were temperature 190℃ , sulfuric acid concentration 10 g/L, pretreatment time 3 min and liquid to solid ratio 20. Under these conditions the sugar yields were 44.6% in pretreatment and 30.3% in enzymatic hydrolysis. On the other hand, with microwave-assisted sodium hydroxide pretreatment the optimal conditions were temperature 130℃ , sodium hydroxide concentration 15 g/L, pretreatment time 7 min and liquid to solid ratio 30. Under these conditions the sugar yields were 1.5% in pretreatment and 80.0% in enzymatic hydrolysis.