茶饮料中糖精钠的测定

Determination of saccharin sodium salt in red tea drink by fluorimetry

建立了茶饮料中糖精钠的荧光分光光度测定方法。样品前处理采用盐酸酸化,加入氯化钠,乙醚萃取后再用2 g/L碳酸钠溶液洗涤乙醚萃取层,加热除去乙醚定容后用荧光光度计测定。测试条件为:激发波长265 nm,发射波长475 nm。方法检测限为2 mg/kg,线性范围在2～60 mg/kg之间,变异系数为1.97%,回收率96.8%。

The method for determination of saccharin sodium salt by fluorimetry was established. The pretreatment of sample included hydrochloric acid aeidized, sodium hydroxide added, ethylether extracted and washed by 2 g/L soda ash solution, ethylether residue removed by heating and the sample was tested after volume was adjusted. The test condition were： excitation wavelength was 265 nm, emission wavelength was 275 nm. The detection limit is down to 2 mg/kg and the linear range is between 2-60 mg/kg, the relative standard deviation is 1.97%, the recovery is 96.8%.