鼻咽癌组织中APE和HIF-1α表达的临床意义:

The Clinical Significance of APE and HIF-1α Expression in Nasopharyngeal Carcinoma

目的:研究无嘌呤/无嘧啶内切核酸酶(APE)和乏氧诱导因子1α(HIF-1α)表达与鼻咽癌生物学行为的关系,探讨其在鼻咽癌诊断和治疗中的价值。方法:应用免疫组化技术,检测47例鼻咽癌和20例鼻咽慢性炎性组织中APE和HIF-1α的表达,并对10例鼻咽癌患者放疗前和放疗期间组织中APE和HIF-1α表达水平进行动态检测。结果:鼻咽癌组织中APE的表达高于鼻咽慢性炎性组织,但其差异无统计学意义;APE表达与性别、年龄、T分期、有无颈淋巴结转移和临床分期均无关。鼻咽癌组织中HIF-1α的表达显著高于鼻咽慢性炎性组织;HIF-1α表达与T分期、有无颈淋巴结转移和临床分期相关,但与性别、年龄无关;放疗后获得肿瘤局部控制的患者,其放疗期间HIF-1α表达水平显著低于放疗前。鼻咽癌中APE和HIF-1α的表达未见相关性。结论:APE和HIF-1α在鼻咽癌中均呈过表达,提示两者均可能涉及鼻咽癌的发生、发展过程。HIF-1α表达水平的检测对鼻咽癌的诊断、分期及疗效判断有重要价值。

Objective： To investigate the correlation of the expression of Apurinic/apyrimidinic endonuclase （APE） and Hypoxia-inducible Factor la （HIF-1α） with the clinical biological behavior of nasopharyngea carcinoma （NPC）, and to explore the value of detecting these two indices in the diagnosis and treatment of NPC. Methods： The expression of APE and HIF-1α was examined with immunohistochemistry in 47 samples of NPC and 20 samples of chronic nasopharynxitis. The dynamic variations in the expression levels of APE and HIF-1α before radiotherapy （pre-RT） and during radiotherapy （dudng-RT） were detected in 10 cases of NPC patients. Results： The expression of APE in NPC was not significantly different from that in chronic nasopharynxitis. There were no correlations between APE expression and gender, age, T-stage, nodal involvement and clinical stage. HIF-1α expression in NPC was significantly higher than that in chronic nasopharynxitis. There were significant correlations between HIF-1α expression and T-stage, nodal involvement and clinical stage. No correlation was found between HIF-1α expression and gender and age. Expression levels of HIF-1α in patients during RT treatment was significantly decreased compared with that before RT treatment. There was no significant correlation between APE expression and HIF-1α expression in NPC. Conclusion： Overex- pression of APE and HIF-1α was common in NPC, and may be associated with the carcinogenesis and development of NPC. Detection of HIF-1α expression level is of great value in diagnosing and staging NPC and is helpful for the evaluation of therapeutic effect of treatment for NPC.