胸腺基质淋巴生成素在正常和炎性牙龈组织中的表达

Local Expression of Thymic Stromal Lymphopoietin in Healthy and Inflamed Human Gingiva

目的:观察胸腺基质淋巴生成素(TSLP)在正常和患牙周炎的牙龈组织中的表达。方法:采用免疫组化和Western blot方法检测20例未经治疗的牙周炎牙龈和8例临床健康牙龈组织中TSLP的表达情况。结果:正常牙龈组织中TSLP在上皮全层均有表达,上皮下结缔组织中仅有散在的弱表达。在炎症牙龈中,TSLP在上皮层强表达,在结缔组织的成纤维细胞,炎性细胞和血管内皮细胞中呈强弱不等的表达;与正常牙龈相比,TSLP蛋白水平显著上调(P<0.05)。结论:TSLP在正常和炎性牙龈组织中均表达,但在患牙周炎的炎性牙龈中表达显著增强,可能在牙周炎的炎症免疫破坏过程中发挥一定作用。

Objective： To investigate the expression of the thymic stromal lymphopoietin （TSLP） in healthy and inflamed human gingival tissues. Methods： Eight healthy subjects and 20 patients with untreated periodontitis were recruited and their gingival biopsies were collected. The expression of TSLP was detected with immunohistochemical staining and Western blot. Results： TSLP peptide was detected in all of the samples. In healthy subjects, TSLP was confined to the gingival epithelium. In patients, the expression of TSLP was extended from the gingiva epithelial to the underlying connective tissue, especially concentrated on the dense inflammatory cells. The TSLP protein were detected in both healthy and inflamed gingival, but the level of TSLP in inflamed gingiva was higher than that in healthy control （P〈0.05）. Conclusion： This study showed the expression of TSLP in gingival tissues and found that TSLP was significantly elevated in inflamed gingiva of periodontitis patients than that of healthy. TSLP might play an important role in the pathological process of periodontitis.