雌兔泌尿生殖系统动脉血管网模型的构建

Construction of arterial network model of female rabbit genitourinary system

目的探讨应用不同浓度灌注液、不同灌注通道构建雌兔泌尿生殖系统动脉血管网模型的方法,为数字化人体泌尿生殖系统动脉血管网模型的构建提供技术基础。方法配制浓度分别为20%、10%和8%的过氯乙烯灌注液,分别经腹主动脉(A组)和腹主动脉+股动脉(B组)进行灌注,制成雌兔泌尿生殖系统动脉血管网铸型标本后,观察评估其血管的表达。结果以不同浓度灌注液及灌注通道构建的雌兔泌尿生殖系统动脉血管网模型,其动脉血管网的表现效果有明显差异:(1)高浓度(A、B)组显示的动脉分支有明显中断现象,填充剂分布不均匀,主要显示大的动脉主干,细小动脉分支难以显示;(2)中浓度(A、B)组能清晰显示动脉主干走行及分布,血管网较完整且显示清晰,分支无明显断裂现象,但B组血管网显示更饱满;(3)低浓度(A、B)组灌注后能清晰显示动脉主干走行,但末梢动脉血管网显示过密,部分呈聚集现象,分层显示不够清晰。结论采用10%浓度的过氯乙烯灌注液,分别经腹主动脉和股动脉同时灌注,能清晰显示完整饱满、分支无明显断裂的血管网。

Objective To explore the method with different concentrations of perfusion fluid and different ways of infusion in construction of the arterial network model of female rabbit genitourinary system, trying to provide some technical foundation for the arterial network of digital human genitourinary system. Method Three different concentrations of chlorinated poly vinyl chloride, 20%, 10% and 8% respectively, were prepared for perfusion fluid to construct the female rabbit genitourinary arterial network system through the abdominal aorta （group A） and abdominal aorta and bilateral femoral artery （group B） which were two ways of infusion, and then to analyze the expression of the cast specimens＇ blood vessels. Result With different concentrations of fillers and through different ways of infusions, the arterial vascular network models of female rabbit genitourinary system showed a significant difference in performance： （1） the model of high concentration group resulted in obvious interrupted branches and uneven distribution. It could only show the main arteries, but imperfectly for the small artery branches showing, （2）the model of middle concentration group could show the main arterial course with no significant im perfect branches; and the distribution of vascular network is full and clear： （3） the model of low concentration group could show the main arteries clearly but too many peripheral or distal branches had overmuch gathered together. Conclusions The model constructed with the 10% vinyl chloride infusion liquid and through the abdominal aorta and bilateral femoral arteries simultaneously could clearly display the full arterial vascular network.