腺病毒介导hIL-24基因对瘢痕疙瘩表达Bcl-2等细胞因子的影响

Effects of recombinant adenovirus-mediated human Interleukin-24 gene on expression of Bcl-2,IL-6 and TGF-β_1 of keloids

目的:研究重组腺病毒介导hIL-24基因对瘢痕疙瘩成纤维细胞表达Bcl-2、IL-6和TGF-β1的影响,探讨IL-24基因对瘢痕疙瘩的生物学作用。方法:体外培养人正常皮肤及瘢痕疙瘩成纤维细胞,用含有hIL-24基因的腺病毒载体感染瘢痕疙瘩成纤维细胞,应用双抗体夹心酶联免疫吸附试验(ABC-ELISA)检测人正常皮肤及瘢痕疙瘩成纤维细胞中的Bcl-2、IL-6和TGF-β1的表达水平。结果:瘢痕疙瘩组的Bcl-2、IL-6和TGF-β1表达水平明显高于正常皮肤组;瘢痕疙瘩感染Ad.IL-24组的Bcl-2、IL-6和TGF-β1表达水平都低于腺病毒空载体组和瘢痕疙瘩组。结论:IL-24基因可抑制瘢痕疙瘩成纤维细胞表达Bcl-2、IL-6和TGF-β1。

Objective Previous studies have shown that IL-24 as a candidate of tumor suppressor gene has tumorsuppressor activity in a broad spectrum of human cancer cells both in vitro and in vivo. In this study, we investigate the effects of recombinant adenovirus-mediated human interleukin-24 gene on expression of Bcl-2, IL-6 and TGF-β1 of kelcid fibroblasts, and the biological role of human IL-24 gene in keloids, to provide a basic theory for gene therapy of keloids. Methods Fibroblasts isolated from human normal skin and keloids were cultured in DMEM medium with 10% fetal bovine serum for 24, 48, 72 hours. The expression levels of BcI-2, IL-6 and TGF-β1 in the supernatants were detected by ABC （avidin-biotin-peroxidase complex）-ELISA. Results At different times, the expression levels of Bcl- 2,1L-6 and TGF-β1 in the supernatants from keloids group were higher than human normal skin group obviously; the expression levels of Bcl-2, IL-6 and TGF-β1 in the supernatants from keloids infected Ad.lL-24 group were lower than keloids infected Ad.GFP group and keloids group. Conclusions Human IL-24 gene can inhibit expression of Bcl-2, IL-6 and TGF-β1 of cultured keliod fibroblasts.