网络抗氧化剂对非酒精性脂肪性肝炎大鼠氧化应激作用的影响

Oxidative stress effects of network antioxidants on nonalcoholic steatohepatitis of rats

目的探讨网络抗氧化剂对非酒精性脂肪性肝炎(NASH)大鼠氧化应激作用的影响。方法雄性SD大鼠30只,随机分为3组,正常对照组、模型组、网络抗氧化剂组。正常对照组普通饲料喂养,模型组喂高脂饮食,网络抗氧化剂组在高脂饮食12周后给予维生素E(100mg/kg)、维生素C(100mg/kg)、硫辛酸(40mg/kg)、辅酶Q10(100mg/kg)、还原性谷胱甘肽(100mg/kg)混合灌胃治疗。16周末处死各组大鼠,测定血清转氨酶(ALT、AST)及硒-谷胱甘肽过氧化物酶(Se-GSH-PX)活性,光镜下观察肝脏组织病理形态学改变,测定肝组织丙二醛(MDA)含量及超氧化物歧化酶(SOD)活性。结果与正常对照组比较,模型组大鼠血清ALT、AST显著升高(P<0.05);肝组织MDA含量增高(P<0.05),SOD及Se-GSH-PX活性下降(P<0.05);肝脏脂肪变性程度和炎症活动度均有显著增高(P<0.05),与模型组比较,网络抗氧化剂组可促进上述指标恢复(P<0.05)。结论网络抗氧化剂对高脂饮食诱导的大鼠NASH有一定治疗效果,网络抗氧化剂之间协同增强的抗氧化能力有效阻遏了自由基引发的氧化应激,保持了氧化/抗氧化平衡,改善NASH大鼠脂肪变性,减轻炎症反应。

Objective To investigate oxidative stress effects of network antioxidants on nonalcoholic steatohepatitis （NASH） of rats. Methods Thirty male rats were randomly divided into normal control group （ordinary diet）, model group（high-fat diet） , network antioxidants therapeutic group （intragastric administration with vitamin E 100 mg/kg, vitamin C 100 mg/kg, lipoic acid 40 mg/kg, coenzyme Q10 100 mg/kg, and glutathione 100 mg/kg, after feeding high-fat diet 12 weeks）. The rats in every group were killed at 16 weeks. Blood samples were collected for the detection of serum alanine aminotransferase （ALT）, aspartate transaminase （AST）, and selenium-glutathione-peroxidase （Se-GSHPX）. The histological changes were observed under light microscope. Liver tissues were obtained for detection of malondistdehyde （MDA） contents, superoxide dismutase （SOD） activity. Results Compared with normal control group, the levels of ALT and AST, and the liver tissue contents of MDA increased （P 〈 0.05）, but SOD and Se-GSH-PX decreased significantly （P 〈 0.05） in model group. The severity degree of hepatic fatty degeneration aggravated and inflammation score significantly increased in model group. Compared with model group, the markers were all markedly resumed in the network antioxidants therapeutic group. Conclusion Network antioxidants can treat NASH of rats induced by high-fat diet. The synergetic enganced antioxidant ability of network antioxidants can stop oxidative stress caused by free radical effectively, keep the balance of oxidation and anti-oxidation, improve the fatty degeneration of NASH of rats, and alleviate the inflammatory reaction.