L-型钙通道α-1c亚型mRNA在结石胆囊组织的表达

Expressions of Cav1.2 mRNA in different types of gallstone gallbladder tissues

目的研究不同类别结石胆囊组织L-型钙通道(Cav1.2)mRNA的表达,探讨胆囊结石形成的可能分子机制及意义。方法应用RT-PCR半定量法分析胆色素结石、胆固醇结石、胆囊息肉的胆囊组织Cav1.2mRNA的表达量。结果Cav1.2mRNA的表达在胆色素结石组和胆囊息肉组明显高于胆固醇结石组(P<0.05),但在胆色素结石组和息肉胆囊组之间差异无统计学意义(P>0.05)。结论Cav1.2mRNA在胆色素结石、胆固醇结石、胆囊息肉三组胆囊组织中均有表达,并且在不同组别中的表达存在明显的差异,这种差异可能是钙离子通道在各类胆囊上分布差异的分子基础,在胆囊结石形成过程中起着重要作用。

Objective To investigate the expressions of Cav1.2 mRNA in pigment gallstones, cholesterol gallstones, and gallbladder polyps, and to explore the possible molecular mechanism and significance of gallstone formation. Methods The expressions of the α-subunit Cav1. 2 mRNA were analyzed by semiquantitative RT-PCR in pigment gallstones, cholesterol gallstones and gallbladder polyps. Results The expressions of Cav1.2 mRNA were higher in pigment gallstones and gallbladder polyps than that in cholesterol gallstones （P 〈 0.05） , but there was no significant difference between pigment gallstones and gallbladder polyps （P 〉 0.05）. Conclusion The results demonstrate that Car1. 2 mRNA expresses in pigment gallstones, cholesterol gallstones and gallbladder polyps. There is a obvious difference in three groups, the difference may be the molecular mechanism of Cav1.2 mRNA distributed in different types of gallbladder tissues, it may play an important role in the progress of gallstones.