不同品系来源的骨髓间充质干细胞对小鼠肾缺血-再灌注损伤作用的对比性研究

Effect of bone marrow mesenchymal stem cells on the recovery of renal ischemia-reperfusion injury in different strain mice

目的探讨不同品系的骨髓间充质干细胞(BMSCs)对各自小鼠肾缺血-再灌注损伤是否有促修复作用,为临床治疗肾脏疾病提供思路。方法取健康3w龄C57BL/6J小鼠和ICR小鼠的骨髓细胞悬液,进行BMSCs体外扩增培养。将8w龄C57BL/6J小鼠和ICR小鼠各随机分为假手术组、平行对照组和细胞移植组,每组12只小鼠。建立肾缺血-再灌注损伤模型,将BMSCs移植到细胞移植组小鼠中,平行对照组注射生理盐水。2w后,检测肾功能和肾形态学变化,观察不同品系的BMSCs对各自小鼠肾缺血-再灌注损伤是否有促修复作用。结果分离培养的BMSCs增殖旺盛,纯度较高,且均质性和稳定性好。BMSCs移植C57BL/6J小鼠后,细胞移植组小鼠整体状态良好,与平行对照组相比肾功能指标明显改善(P<0.05),组织形态学好转。而BMSCs移植ICR小鼠后,细胞移植组小鼠整体状态差,肾功能指标与平行对照组相比没有差异或者明显高于平行对照组(P>0.05),肾病理性改变没有得到改观。结论经尾静脉移植BMSCs后,可提高肾缺血-再灌注损伤后宿主C57BL/6J小鼠的恢复;但不能改善ICR小鼠肾缺血-再灌注造成的损伤,甚至加剧了ICR小鼠肾损伤的程度。

Objective To explore whether bone marrow mesenchymal stem cells （BMSCs） from different strain mice can promote repairing renal ischemia-reperfusion （I/R） injury and provide a method for the treatment of renal diseases. Methods BMSCs were cultured and expanded from healthy 3-week-old C57BL/6J mice and ICR mice in vitro, respectively. 8-week-old C57BL/6J mice and ICR mice were randomly divided into sham, parallel control and cell transplantation groups, and 12 mice in each group. The renal I/R injury model was established. BMSCs were transplanted into cell transplantation group mice via the tail vein and saline was injected into parallel control group. Then, renal function, morphologic changes and renal pathology changes were detected after 2 weeks. The recovery of kidney of different strains mice were observed. Results BMSCs not only had a high purification, homogeneity and stability, but also had unlimited prolifera- tion. The 57BL/6J mice in transplantation group appeared the good state, and the indexes of renal function were significantly improved （P 〈 0. 05 ）, the renal histomorphology became better. The ICR mice in transplantation group appeared the poor state, the indexes of renal function were not different from those in control group, and some indexes were significantly higher than those of control group （ P 〉 0. 05 ）, the renal pathological changes were not improved. Conclusions The recovery of renal I/R injury in the host is improved after transplantation of BMSCs into C57BL/6J mice by tail vain and renal injury is improved. But BMSCs can not improve ICR mice renal I/R injury, or even exacerbate the extent of renal injury.