摘要
目的:探讨舌鳞癌(TSCC)中细胞质膜微囊蛋白-1(caveolin-1)基因及蛋白的表达水平及其与肿瘤生物学特性的关系。方法:应用原位杂交和免疫组化法,测定92例TSCC患者舌体癌组织及癌周正常舌组织中caveolin-1基因及其蛋白表达水平变化,应用SPSS10.0软件包对数据进行统计学分析。结果:TSCC中caveolin-1基因mRNA表达水平(24.6±1.8)及蛋白水平(471.2±68.1)分别低于正常组织的167.2±20.5及964.5±77.2,差异均有统计学意义(P<0.01)。高分化TSCC中,caveolin-1蛋白表达水平为5.6±1.4,高于中、低分化的1.3±0.8,差异有统计学意义(P<0.01);临床Ⅰ~Ⅱ期表达水平为6.1±2.0,高于临床Ⅲ~Ⅳ期的2.4±0.7,差异有统计学意义(P<0.01)。颈淋巴结转移与caveolin-1蛋白表达水平无关(P>0.05)。结论:TSCC组织中caveolin-1基因及蛋白的表达明显下调,caveolin-1蛋白的表达与肿瘤的分化程度、临床分期有关,而与肿瘤转移无关。
PURPOSE: To investigate the changes and the significance of caveolin-1 gene and protein in tongue squamous cell carcinoma(TSCC). METHODS: Immunohistochemieal staining and in situ hybridization were used to detect the changes of caveolin-1 gene and the protein in 92 TSCCs and their adjacent normal tissues. The data were analyzed with SPSS 10.0 software package. RESULTS: The expressions of caveolin- 1 gene (24.6± 1.8 ) and its protein (471.2±68.1 ) in TSCC were lower than those of the control group(167.2±20.5, 964.5±77.2), respectively. The difference was significant (P〈0.01). The expression of eaveolin-1 protein in well-differentiated TSCC(5.6±1.4) was higher than that in moderately and poorly differentiated TSCC (1.3±0.8); The expression of caveolin-1 protein in stage Ⅰ-Ⅱ patients(6.1±2.0) was higher than that in stage Ⅲ-Ⅳ patients (2.4±0.7), and the difference was significant (P〈0.01). There was no significant relationship between the expression of caveolin-1 protein and cervical lymph node metastasis (P〉0.05). CONCLUSIONS: The level of caveolin-1 gene and the expression of the protein decrease in TSCC and the level of protein has a significant relationship with the clinical stage and the pathological grade, rather than the cervical lymph node metastasis. Supported by Science and Technology Research Project of Liaoning Province(Grant No. 2005225007-1).
出处
《上海口腔医学》
CAS
CSCD
2009年第3期243-246,共4页
Shanghai Journal of Stomatology
基金
辽宁省科学技术计划项目(2005225007-1)~~