结肠癌组织中PTEN蛋白表达与PI3K/AKT信号通路的关系

The Relationship Between PTEN Expression and PI3K/AKT Signal Pathway in Colon Carcinoma

目的探讨抑癌基因PTEN与结肠癌发生发展的关系及其与PI3K/AKT信号传导通路的相关性。方法应用Western blot蛋白印迹技术和免疫组织化学方法检测32例结肠癌及癌旁正常组织中PTEN和p-AKT蛋白的表达。结果PTEN蛋白在结肠癌中表达的阳性率(31.25%)低于癌旁正常组织(96.87%)(P<0.01);而p-AKT蛋白在结肠癌中表达的阳性率(87.5%)高于癌旁正常组织(15.62%)(P<0.01);两种蛋白的表达呈负相关(r=-0.872,P<0.01),且表达水平与肿瘤的分化程度、Duke’s分期、有无淋巴结转移及远处器官转移有关(P<0.05)。结论PTEN蛋白表达缺失导致AKT信号传导通路持续活化可能在结肠癌的发生发展过程中起着重要作用。

Objective To investigate the expression of PTEN in carcinogenesis and development of colon carcinoma and to reveal their correlation with the PI3K/AKT pathway. Methods The expressions of PTEN and p-AKT were detected by immunohistoehemistry and western blotting in 32 specimens from patients with colon carcinoma. Results The positive rate of p-PTEN was significantly lower in colon carcinoma （31.25%） than that in normal control tissues （96. 87%, P〈 0. 01）, and the positive rate of p-AKT was significantly higher in colon carcinoma （87.5%） than that in normal control tissues （15.62% ,P〈0.01）, and expression level of PTEN and p-AKT were related to histological differentiation, distant invasion, lymph node metastasis and Duke＇s stage （P〈 0. 05）. A negative correlation was observed between the expression of PTEN and p-AKT proteins. （r=-0. 872, P〈0.01）. Conclusion The over-expression of AKT accompanied by the loss of PTEN involves carcinogenesis and development of colon carcinoma.