蒿甲醚-蔗糖铁脂质纳米粒的制备及表征

Preparation and Characterization of Artemether-iron Sucrose Lipid Nanoparticles

目的研制蒿甲醚-蔗糖铁脂质纳米粒且表征其理化性质。方法以大豆卵磷脂和大豆油为载体,采用薄膜分散-高压乳匀法制备蒿甲醚-蔗糖铁脂质纳米粒。用高效液相色谱法测定包封率和载药量,用电子显微镜观察形态,用激光粒度仪测定粒径。结果制得的脂质纳米粒呈类球状,粒径较均匀,平均粒径为(161±17.4)nm,包封率为(85.1±0.83)%,载药量为(5.3±0.2)%。体外释放实验表明,脂质纳米粒具有良好的缓释特征。结论该制备工艺简单可行,制得的纳米粒分散均匀,包封率较高。

Objective To prepare Artemether-Iron Sucrose lipid nanoparticles and examine the characteristics of the nanoparticles. Methods The Artemether-Iron Sucrose lipid nanoparticles were prepared by film-dispersing and high-pressure homegenization technique using soybean phospholipid and soybean oil as matrix. The entrapment efficiency and drug loading capacity were determined by HPLC. The morphology was examined by transmission electron microscope and the mean particle size was measured by laser light scattering technique. Results The products were spherical and well distributed. The mean diameter was （161 ± 17.4） nm with （85. 1± 0. 83）% of entrapment efficiency and （5, 3±0. 2）% of drug loading. The in vitro release test showed that the Art-IS-NLC had good sustained release characteristics. Conclusion The preparation method of Art-IS-NLC is simple and feasible. The products are well distributed with a high entrapment efficiency.