锑对小鼠肝线粒体抗氧化酶系统影响的实验研究

An Experimental Study on Effects of Antimony on Anti-oxidase System on Liver Mitochondria in Mice

目的探讨锑中毒性肝损害的机理。方法本实验以亚急性中毒实验模型，观察了三氧化二锑对小鼠肝线粒体抗氧化酶的影响。结果每天腹腔注射４０ｍｇ／ｋｇ三氧化二锑２８天，染锑动物血清天冬氨酸氨基转移酶和丙氨酸氨基转移酶活性明显增高；肝线粒体超氧化物歧化酶和谷胱甘肽过氧化物酶的活性与对照组比较，差异有显著性；与对照组相比，染毒２８天时，染锑组动物肝线粒体丙二醛为４７２７±１２１μｍｏｌ／Ｌ，与对照组比较，差异有极显著性。结论表明锑引起肝损害的机理与其损伤肝线粒体的抗氧化能力，产生脂质过氧化作用有关。

Objective To explore the mechanisms of toxic liver damage caused by antimony. Methods Experimental mice were injected peritoneally 40 mg/kg of antimony trichloride daily for 28 days to observe the effects of it on the antioxidase system in their liver mitochondria. Results Activities of aspartate aminotransferase and alanine aminotransferase increased obviously in the experimental animals, and those of superoxide dismutase and glutathione peroxidase in liver mitochondria lowered, as compared to those in the control animals with statistical significance. Content of malonyl dialdehyde in the liver mitochondria was 47.27±1.21 μmol/L in the experimental animals, significantly higher than that in controls. Conclusion It suggested that the mechanism of liver damage caused by antimony associated with injury to the ability of antioxidation and lipid peroxidation in the liver mitochondria.