期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

大豆蛋白质相关基因SNP的开发和遗传定位 被引量：3

SNP discovery and linkage analysis of protein related genes in Soybean
下载PDF
导出
摘要 在大豆蛋白质相关基因中利用直接测序法在2个代表性品种Pureukong与Jinpumkong 2之间发掘SNP标记,并利用这些标记将蛋白质相关基因定位在遗传图谱上.为了开发SNP分子标记,选择56个蛋白质相关基因并设计相应的引物.结果显示,49个引物获得较好的测序结果.其中,7个引物在2个亲本之间显示多态性,共检测出11个SNP分子标记.2个亲本间SNP的检测频率为1 SNP/2400 bp.利用杂交获得的重组自交系,大豆蛋白质相关基因被定位到已经构建的以SSR分子标记为主的遗传图谱上. Found SNPs between Purenkong and Jinpumkong 2 using direct sequencing method and to map these genes to soybean linkage map. To discover SNPs, a total of 76 protein related genes were selected and primers were designed. Among 49 loci showing high-quality sequence, 11 SNPs were identified in 7 loci between the parental genotypes. Average SNP frequency between the parental genotypes was one SNP per 2 400 bp. With SNP genotyping in 90 RILs, these genes were mapped in exiting linkage map.
作者 蔡春梅 柳伟先 李锡河
机构地区 青岛农业大学生命科学学院 韩国首尔大学农业与生命科学学院
出处 《延边大学农学学报》 2009年第2期77-83,共7页 Agricultural Science Journal of Yanbian University
基金 韩国科技部支撑项目(CG3121)
关键词 大豆 SNP 蛋白质 Soybean SNP protein
分类号 S565.1 [农业科学—作物学]
  • 相关文献

参考文献18

  • 1Keim P,Diers B W,Olson T C,et al.RFLP mapping in soybean:association between marker loci and variation in quantitative traits[J].Genetics,1990,126:735-742.
  • 2Shoemaker R C,Specht J E.Integration of the soybean molecular and classical genetic linkage groups[J].Crop Sci,1995,35:436-446.
  • 3Cregan P B,Jarvik T,Bush A L,et al.An integrated genetic linkage map of the soybean[J].Crop Sci,1999,39:1464-1490.
  • 4Song Q J,Marek L F,Shoemaker R C,et al.A new integrated genetic linkage map of the soybean[J].Theor Appl Genet,2004 109:122-128.
  • 5Andersen J R,Lubberstedt T.Functional markers in plants[J].Trends Plant Sci,2003,8:554-560.
  • 6Sachidanandam R,Weissman D,Schmidt S C,et al.A map of human genome equence variation containing 1.42 million single nucleotide polymorphisms[J].Nature,2001,409:928-933.
  • 7Cho R J,Mindrinos M,Richards D R,et al.Genome-wide mapping with biallelic markers in Arabidopsis thaliana[J].Nat.Genet,1999,23:203-207.
  • 8Sanchez-Villeda H,Schroeder S,Polacco M,et al.Development of an integrated laboratory information management system for the maize mapping project[J].Bioinformatics,2003,19:2022-2030.
  • 9Rostoks N,Mudie S,Cardle L,et al.Genome-wide SNP discovery and linkage analysis in barley based on genes responsive to abiotic stress[J].Mol Genet Genomics,2005,274:515-527.
  • 10Van K,Hwang E Y,Kim M Y,et al.Discovery of single nucleotide polymorphisms in soybean using primers designed from ESTs[J].Euphytica,2004,139:147-157.

二级参考文献40

  • 1王春娥,盖钧镒.大批量小样品豆腐与豆乳得率的定量分析技术研究[J].大豆科学,2007,26(2):223-229. 被引量：9
  • 2张学勇,童依平,游光霞,郝晨阳,盖红梅,王兰芬,李滨,董玉琛,李振声.选择牵连效应分析:发掘重要基因的新思路[J].中国农业科学,2006,39(8):1526-1535. 被引量：71
  • 3卢为国,盖钧镒,郑永战,李卫东.大豆遗传图谱的构建和抗胞囊线虫(Heterodera glycines Ichinohe)的QTL分析[J].作物学报,2006,32(9):1272-1279. 被引量：18
  • 4Suh H S, Hue M H. The segregation mode of plant height in the cross of rice varieties. XI. Linkage analysis of the semi-dwarfness of the rice variety 'Tongil'. Korean Journal of Breeding, 1978, 10:1-6.
  • 5Allan R E. Differentiating between two Norin 10/Brevor 14 semi-dwarf genes in a common genetic background. Seiken Ziho, 1970, 22:83-90.
  • 6Tanksley S D, McCouch S R. Seed banks and molecular maps: unlocking genetic potential from the wild. Science, 1997, 277: 1 063-1 066.
  • 7Rajaram S, van Ginkel M. Yield potential debate: Germplasm vs. methodology, or both. In Reynolds M P, Rajaram S, McNab A (eds.) Increasing the Yield Potential in Wheat: Breaking the Barriers. Mexico D.F.: CIMMYT. 1996: 11-18.
  • 8Yu J, Hu S N, Wang J, Wong G K S, Li S G, Liu B, Deng Y J, Dai L, Zhou Y, Zhang X Q, Cao M L, Liu J, Sun J D, Tang J B, Chen Y J, Huang X B, Lin W, Ye C, Tong W, Cong L J, Geng J N, Han Y J, Li L, Li W, Hu G Q, Huang X G, Li W J, Li J, Liu Z W, Li L, Liu
  • 9Feng Q, Zhang Y J, Hao P, Wang S Y, Fu G, Huang Y C, Li Y, Zhu J J, Liu Y L, Hu X, Jia P X, Zhang Y, Zhao G, Ying K, Yu S L, Tang Y S, Weng Q J, Zhang L, Lu Y, Mu J, Lu Y Q, Zhang L S, Yu Z, Fan D L, Liu X H, Lu T T, Li C, Wu Y R, Sun T G, Lei H Y, Li T,
  • 10Sun X L, Cao Y L, Yang Z F, Xu C G, Li X H, Wang S P, Zhang Q F. Xa26, a gene conferring resistance to Xanthomonas oryzae pv. oryzae in rice, encodes an LRR receptor kinase-like protein. The Plant Journal, 2004, 37:517-527.

共引文献85

同被引文献71

引证文献3

二级引证文献5

延边大学农学学报
2009年 第2期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部