梨轮纹病菌培养特性研究

Study on the specific method for culturing Botryosphaeria berengeriana f. sp. piricola

研究了梨轮纹病原菌的培养特性，结果表明，不同病原菌菌株间的致病力、菌丝日生长速率及产孢能力均不同，生长速率最大的菌株为P-16，生长速率最小的菌株为P-10，差异较大。菌株P-16致病力最强，菌株P-10致病力最弱。菌株P-7、P-13、P-16产孢量较大，P-4、P-10、P-19无分生孢子器产生；病原菌菌丝最佳生长温度为28℃，最佳产孢温度为25℃，分生孢子最佳萌发温度28℃；在日光灯、黑光灯条件下有利于病原菌菌丝生长，黑暗条件下病原菌生长较慢；病原菌产孢在黑光灯条件下最好；不同光照条件对病原菌分生孢子萌发影响不大：病原菌菌丝生长最适pH值为7-9，产孢最适pH值为6-8，分生孢子萌发最适pH值为7-9；病原菌菌丝致死温度为62℃（10min）或57℃（15min），分生孢子致死温度为60℃（10min）或55℃（15min）。

The specific method for culturing Botryosphaeria berengeriana f. sp. piricola was studied. The resuhs showed that different strains had different growth rate,pathogenicity and sporulation quantity. P-16 had the maximum daily growth rate and P-10 had the least; P-16 had the most powerful pathogenicitiy and P-10 had the least; P-7 ,P-13 ,P-16 had the largest Sporulation quantity, but P-4, P-10, P-19 had no pycnidium .The optimum temperatures for mycelium growth, sporulation and conidial germination were 28℃, 25℃ and 28℃, respectively. Daylight lamp and Dark light lamp was beneficial to mycelium growth, dark conditions were against mycelium growth ; whereas black light lamp condition could promote sporulation. However,the conidial germination showed to be less affected by light conditions. The proper pH values for mycelium growth,sporulation and conidial germination were 7-9, 6-8 and 7-9, respectively. The lethal temperatures for mycelium and eonidiospore were 62℃（10min） or 57℃（15min）, and 60℃（10min） or 55℃（15min）.