摘要
目的:观察三七对酒精性脂肪肝大鼠血清TNF-α、IL-6、IL-8的水平和肝组织TNF-α、IL-6、IL-8基因mRNA表达的影响,探讨三七对酒精性脂肪肝大鼠肝脏损害保护效应的作用机制。方法:62只大鼠随机分为正常组10只,模型组、易善复组、三七大、小剂量组各13只;模型组大鼠2次/d采用50%酒精灌胃,同时每天自由饮用10%酒精饮料进行造模,易善复组、三七大、小剂量组大鼠在造模的同时予相应的药物进行干预,连续14周后观察肝组织的病理学改变,放免法测定血清中TNF-α、IL-6、IL-8含量,原位杂交检测肝组织中TNF-α、IL-6、IL-8的mRNA表达。结果:模型组大鼠肝组织出现不同程度的肝脂肪变性,血清中TNF-α、IL-6、IL-8含量和肝组织TNF-αmRNA、IL-6mRNA、IL-8mRNA表达较正常组明显增高,应用三七大、小剂量干预后,大鼠肝组织脂肪变明显减轻,血清TNF-α、IL-6、IL-8含量和肝组织TNF-αmRNA、IL-6mRNA、IL-8mRNA表达明显降低。结论:长期单纯大量饮酒可造成大鼠TNF-α、IL-6、IL-8等细胞因子分泌的异常,三七能调节细胞因子网络,抑制酒精性脂肪肝大鼠TNF-α、IL-6、IL-8等的生成可能是其有效防止酒精性脂肪肝发生的重要机制之一。
Objective:To observe the the effect of Panax Notogenseng on the contents of serum TNF -α,IL - 6 and IL - 8 and the expression of TNF -α, IL - 6 and IL - 8 of liver in alcoholic fatty liver rats, to explore the mechanism of Panax Notogenseng,sprotective effect on the liver injury in alcoholic fatty liver rats. Methods:Sixty-two rats were randomly divided into five groups,the normal group( n = 10) ,the model group,the Yishanfu group,the low and the high conceratlon of Panax Notogenseng group( respectively n = 13 ) all the rats in the treatment group were dealed with the 50% alcohol twice every day by garage and drank the 10% alcohol freely. Simultaneously,The rats in the Yishanfu group,the low and high eoneeration of Panax Notogenseng group were dealed with different medicine. At the end of 14th week,all rats were put to death ,the contents of serum TNF -α IL -6 and IL -8 were examined and pathologic change in rats' liver was observed and the expressing of TNF -αmRNA IL - 6mRNA and IL - 8mRNA in hepatic tissue were detected by hybridization in situ. Results :In the model,to different degree,there were some fatty steatosis,the amounts of serum TNF -α IL-6 IL - 8 and the expression ofTNF -α mRNA IL - 6mRNA and IL - 8mRNA in hepatic tissue were higher than'those in the normal group, and were lower than those in the Panax Netogenseng group. Conclusion:Long-term of ingestion of potation maybe cause the secretion abnormity of cytokines, such as TNF -α IL - 6 IL - 8. Panax Netogenseng can regulate cytokine network, restrain the formation of TNF -α IL - 6 IL - 8 in alcoholic fatty liver rats which is the possible important mechanism for Panax Netogenseng to prevent alcoholic fatty liver.
出处
《中华中医药学刊》
CAS
2009年第7期1457-1459,共3页
Chinese Archives of Traditional Chinese Medicine
基金
浙江省中医药科技计划资助项目(2007YA009)
关键词
三七
酒精性脂肪肝
细胞因子
Panax Netogenseng
alcoholic fatty liver
cytokines