左归饮对衰老模型小鼠血清SOD GSH-Px MDA及IL-2的影响

Effect of Zuoguiyin on Activity of SOD and GSH-Px and MDA and IL-2 in Serum of Aged Model Mice

目的:探讨左归饮延缓衰老的作用机制。方法:将50只小鼠随机分为5组,除正常对照组外均每日注射D-半乳糖造成衰老模型,同时给予不同剂量的左归饮。6周后检测小鼠血清中超氧化物歧化酶(SOD)和谷胱甘肽过氧化物酶(GSH-Px)活性,丙二醛(MDA)含量和白细胞介素-2(IL-2)水平。结果:模型对照组与正常对照组比较,小鼠血清中SOD、GSH-Px活性显著降低,MDA含量显著升高,IL-2水平显著下降(P<0.01)。与模型对照组比较,左归饮高、中、低剂量组小鼠血清中SOD活性显著升高(P<0.01,P<0.01,P<0.05),GSH-Px活性显著升高(P<0.01),MDA含量显著降低(P<0.01,P<0.01,P<0.05);左归饮高、中剂量组IL-2水平显著升高(P<0.05)。结论:左归饮可通过抗氧化作用及对IL-2影响延缓衰老。

Objective： To investigate whether Zuognlyin is effective on deferring aging and its mechanism. Methods： Fifty mice were randomly divided into five groups. They were treated with D - galactose for aged model mice except control group,then they were treated with ZGY. The activities of SOD and GSH- Px and MDA and IL-2 in serum of fifty mice were determined and compared before and after treatment. Results ： Significantly, the activity of SOD and GSH-Px and IL - 2 was lower and the content of MDA was higher in aged model group than that in control group（ P 〈 0. 01 ）. Compared with aged model group,the activities of SOD and GSH -Px were significantly increased in ZGY high,middle,low dose groups （ P 〈 0. 05 ）. Meanwhile, the contents of MDA were significantly reduced （ P 〈 0. 05 ）. The levels of IL - 2 were significantly increased in ZGY high, middle,dose groups（ P 〈 0.05 ）. Conclusion ：ZGY can increase the antioxidation activity and IL-2 ,and delay the senility,these functions maybe related with immunoregulation of ZGY.