慢病毒载体介导RNAi的研究进展

Advance in RNAi Mediated with Lentivirus-based Vectors

RNA i通过双链RNA的介导,特异性阻抑相关序列的表达,从而导致转录后水平的基因沉默。广泛存在于真菌、植物和动物等真核生物中。慢病毒载体是理想的真核细胞基因转移工具,被广泛应用于相关的RNA i研究领域,例如抗病毒研究、癌症及其治疗、遗传性疾病的治疗、基因治疗。现已发现,慢病毒载体能够介导组织特异、时间特异的RNA i,在疾病的基因靶向性治疗上必有广阔的前景。

RNA interference is a post-transcriptional gene silencing mechanism, by which double-stranded RNA specifically suppresses the expression of coherent sequences. Consequently, RNA interference can be observed in eukaryotic organisms including fun- gi,plants and animals. As a good eukaryotic cells gene transfer vector, lentivirus-based vectors are generally applied in RNAi-related research, such as antiviral research, research and therapy of cancer, therapy of hereditary diseases and gene therapy. RNAi mediated by lentivirus-based vectors is tissue-specific and time-specific, thus, it would be applied helpfully in targeted gene therapy.