重组运动发酵单胞菌的构建及木糖利用特性研究

The Construction and Characteristic Evaluation of Xylose-utilizing Recombinant Zymomonas mobilis Strain

将大肠杆菌(Escherichia coli)木糖代谢的关键酶基因。引入到运动发酵单胞菌中,获得能利用木糖发酵生产乙醇的重组工程菌株PZM。混合糖发酵过程中,重组菌利用葡萄糖和木糖生成乙醇的效率分别达到理论值的81.2%和63.1%。

Two poerons encoding xylose assimilation and pentose phosphate pathway enzymes from Escherichia coli were constructed and transformed into Z. mobilis. The recombination strain fermentation xylose to ethanol was achieved through a combination of the pentose phosphate pathway and Entner-Doudoroff pathways. PZM can utilize glucose and xylose. The productivity of ethanol from glucose was 81.2% of theory value and 63.1% from xylose.