结直肠癌细胞辐射敏感性的表达谱研究

Study on radiation sensitivity of colorectal cancer cells using gene expression profile

目的筛选结直肠癌细胞辐射敏感相关基因。方法采用人全基因组表达谱芯片获得不同辐射敏感性的两株细胞（Lovo与SW480）之间的基因表达谱．分析比较两者之间基因的差异表达情况。结果Lovo组与SW480组数据比较后．筛选出2倍以上差异表达基因中上调基因908个．其中在Lovo细胞组中较高表达的基因有CEACAM5、THBS1、SERPINE2、ARL7和HPGD，与辐射敏感高度相关；下调基因1312个，其中在SW480细胞中较高表达的基因主要有SCD、NQ01、LYZ、KRT20和ATP1B1．与辐射抵抗有关。结论采用基因方法研究结直肠癌辐射敏感性将更有利于辐射敏感相关基因的筛选。

Objective To screen the radiosensitivity-related genes of colorectal cancer cells. Methods Gene expression profiles of two different radiaosensitivity cells （colorectal cancer cell line Lovo and SW480） were obtained by cDNA array and the differences of gene expression profiles between the two ceils were analyzed. Results Genes of more than 2-fold expressive differentiation were screened. In Lovo cells, 908 up-regulated genes were found, including higher expression genes CEACAM5, THBS1, SERPINE2, ARL7, HPGD, while 1312 genes were down-regulated. In SW480 cells, higher expression genes were SCD, NQ01, LYZ, KRT20 and ATP1B1. Conclusion Gene profiles can reflect the radiosensitivity of colorectal cancer cells, which will provide the choice for the further study of radiosensitivity in colorectal cancer.