摘要
以父母代固始鸡为试验材料,用添加不同剂量的白细胞介素2(IL-2)佐剂的鸡志贺氏菌苗对试验鸡进行分组免疫,应用免疫组织化学技术,通过Qwin图像处理系统对鸡十二指肠内分泌型免疫球蛋白A(SIgA)阳性细胞及其分泌物的分布和阳性面积进行系统分析。结果表明,SIgA阳性细胞主要分布于十二指肠肠黏膜的固有膜及肠腺腔中,在志贺菌组中添加IL-2能够提高SIgA细胞的分泌,从而提高动物体的免疫能力,且以IL-2的添加剂量在50μg时对增加志贺菌苗的免疫性效果最佳。
To investigate the immunological enhancement effects of IL-2 with different doses in chicken Shigella vaccine on the duodenum mucosa in Gushi chicken, parental line Gushi chicken were grouped and inoculated with Shigella vaccines including different dose of IL-2. And then the distribution and change of SIgA positive cells and its secretion in the duodenum of the chicken were observed with the immunohistochemistry technology using the Qwin image analysis system. SIgA postive cells were distributed and located primarily in inhesion membrane and enteraden cavity of the duodenum mucosa, additive IL-2 in the Shigella vaccine could increase secretion of SIgA positive cells and strengthen the immunity of the birds, in some degree. The 50μg IL-2 in the vaccine could display a optimal immunological enhancement effect.
出处
《中国兽医学报》
CAS
CSCD
北大核心
2009年第7期909-913,共5页
Chinese Journal of Veterinary Science
基金
国家"863"计划资助项目(2002AA242021)
