摘要
取单层培养72 h生长良好的犊牛肝细胞,采用单因素重复试验,分别添加0、25、50、100、200、400 ng/L的牛重组抵抗素(resistin),每个处理3个重复(每重复2孔)。继续培养12 h后分别提取RNA并制备细胞上清液。应用荧光定量PCR方法检测牛重组Resistin对肝细胞糖异生关键酶丙酮酸羧化酶(Pyruvate carboxylase,PC)基因表达的影响,同时用比色法检测其对肝细胞PC酶活性的影响。结果表明,一定浓度的resistin显著下调了肝细胞PCmRNA表达,且降低了PC酶活性。
A single factor duplicate test was designed to investigate whether bovine recombinded resistin impacts the expression of pyruvate carboxylase （PC） mRNA and the activity of PC in vitro culture bovine hepatocyte. Bovine recombinded resistin was added to the media with 0,25,50,100,200 and 400 ng/L. Abundance of PC mRNA in bo- vine hepatocyte, which was cultured with bovine recombinded resistin for 12 hours,was determined by real-time fluo- rescence quantitative RT-PCR,and activity of PC was determined by colourimetry. The results showed that bovine reeombinded resistin could downregulate the expression of PC mRNA and the activity of PC in vitro culture bovine hepatocyte.
出处
《中国兽医学报》
CAS
CSCD
北大核心
2009年第7期924-927,共4页
Chinese Journal of Veterinary Science
基金
国家自然科学基金资助项目(30571365)
