摘要
应用清肝化瘀口服液治疗小剂量CCl_4诱导的肝纤维化大鼠，并与正常对照组、CCl_4模型组、自然恢复组、秋水仙碱治疗组对照。对各组肝脏Ⅰ、Ⅲ、Ⅳ、Ⅴ型胶原及结蛋白进行免疫组化染色和计算机图像分析，并用电镜观察肝脏超微结构的变化。结果发现，清肝化瘀口服液可抑制肝细胞变性坏死，减轻炎症反应，改善微循环，抑制Ⅰ、Ⅲ、Ⅳ、Ⅴ型胶原及结蛋白在肝内的沉积，并可使已形成的胶原重新溶解和吸收，使肝纤维化逆转，效果优于秋水仙碱（P＜0．01）；同时，CCl_4诱导的大鼠肝纤维化可有一定程度的自然恢复。表明清肝化瘀口服液对大鼠肝纤维化有显著的治疗作用。
The effect of a herbs mixture, Liver-clearing and Stasis-eliminating mixture(LCSEM) on CCl4-induced hepatic fibrosis of rats was investigated and compared with controlgroup, colchicine group,model group and nontreatment group. Immunohistochemical staining ofⅠ、Ⅲ、Ⅳ、Ⅴ collagen and desmin, computer imaging analysis and electronic microscopy were usedto study the effect. Hepatocyte degeneration and inflammatory reaction were distinctivelyinhibietd,and deposition of type Ⅰ、Ⅲ、Ⅳ、Ⅴ collagen and desmin was significantly decreasedwhile sinusoid microcirculation was restored after LCSEM treatment. This indicates LCSEM hasdissolvent and absorbent efficacy to collagenous fiber which has deposited in the liver. Therefore,hepatic fibrosis might be reversed. The antifibrosis of LCSEM is more effective than colchicine(P ＜0.01 ). Additionally, CCl4-induced fibrosis of rats could somewhat restore. It suggestedthat LCSEM could significantly reduce CCl4-induced fibrosis of rats.
基金
河北省科委科技研究资助!95216124
关键词
清肝化瘀口服液
纤维变性
肝
liver-clearing and stasis-eliminating mixture, hepatic fibrosis, liver
