X染色体相关凋亡抑制蛋白在移行细胞癌中的表达及临床意义

Expression of X-linked inhibitor of apoptosis protein in transitional cell carcinoma and the clinical significance

目的探讨X染色体相关凋亡抑制因子（XIAP）在移行细胞癌（TCC）组织中的表达及意义。方法应用免疫组化S-P法、蛋白质印迹法、RT—PCR法检测43例TCC（肾盂癌6例、输尿管癌2例、膀胱癌35例）以及12例正常移行上皮细胞组织中XIAP蛋白及mRNA表达水平，并采用t检验和方差分析进行统计学比较。结果XIAP蛋白及mRNA表达在TCC和正常移行上皮组织（S-P法：22±5和16±2；蛋白质印迹法：1.21±0．15和0．61±0．24；mRNA：1．17±0．30和0．75±0．17）、膀胱癌复发者与初发者（S-P法：24±3和20±3；蛋白质印迹法：1．66±0．28和1．10±0．23；mRNA：1．44±0．27和1．05±0．23）之间比较差异有统计学意义（P〈0．05）。而在TCC分级、膀胱癌与输尿管和肾盂癌、膀胱癌分期以及膀胱癌单发与多发之间XIAP表达比较差异均无统计学意义（P〉0．05）。结论XIAP在TCC中呈高表达，可能是促进移行上皮恶变的一个重要因素。膀胱癌复发者XIAP表达较初发者明显增高，检测XIAP表达可能成为判断膀胱癌预后、预防膀胱癌复发的新指标。

Objective To study the relationship between X-linked inhibitor of apoptosis protein （XIAP） expression and transitional cell carcinoma（TCC） development. Methods Forty-three TCC tissues and 12 normal transitional epithelial tissues were applied to detect XIAP expression by semiquantitative RT-PCR, immunohistochemistry and western blot. The data were statistically analyzed by using SPSS11. 5 according to the 2 groups （TCC and normal transitional epithelial） as well as the different subgroups （tumor stage, grade, single or multiple tumor, primary or recurrence tumor）. Results XIAP expression in TCC tissues was higher than in normal transitional epithelial tissues（immunohistochemistry：22±5 and 16 ± 2, Western blot： 1.21 ± 0. 15 and 0.61 ± 0.24, mRNA： 1.17 ± 0.30 and 0. 75 ± 0. 17, P〈0. 05）. In the bladder tumors group, XIAP expression in recurrence tumors was higher than in primary tumors （immunohistochemistry： 24 ± 3 and 20 ±3, Western blot： 1.66±0.28 and 1. 10± 0.23, mRNA：1. 44±0.27 and 1.05±0.23, P〈0.05）. However, there were no significant differences according to the tumor stage and tumor grade as well as tumor multi- plicity or not. Conclusion XIAP expression might serve as a biomarker in TCC diagnosis and recurrence prediction.