高压氧对人前列腺癌细胞株小鼠荷瘤模型作用的研究

Effects of hyperbaric oxygen therapy on in vivo murine prostate cancer cell PC-3

目的为评估高压氧治疗前列腺癌放疗后出血性膀胱炎的安全性，探讨高压氧对体内前列腺癌细胞生长的影响。方法采用人前列腺癌PC-3细胞株皮下接种构建小鼠荷瘤模型（n=40），随机分组，实验组（n=20）每周连续进行5次200kPa高压氧暴露，共20次，对照组（n=20）常压常氧条件下饲养。连续4周观察2组移植瘤生长体积的变化，免疫组织化学方法分析2组瘤体组织相关病理学特征，包括瘤体微血管密度（CD34）、瘤细胞增殖（Ki-67蛋白）以及瘤细胞凋亡（p53、p27蛋白）等指标。结果肿瘤接种后第28天，实验组移植瘤体积为（425．8±13．9）mm^3，对照组为（433．6±12．8）mm^3，2组比较差异无统计学意义（P〉0．05）；实验组移植瘤微血管密度及Ki-67、p53、p27蛋白表达的阳性率分别为69．7±9．5、（55．2±6．7）％、（31．2±5．3）％、（80．4±5．7）％，对照组分别为77．1±8．7、（50．64-7．3）％、（30．5±4．7）％、（85．3±6．4）％，2组比较差异均无统计学意义（P〉0．05）。结论高压氧对于前列腺癌细胞生长无促进作用，临床应用高压氧治疗因前列腺癌放射治疗引起的出血性膀胱炎患者可能是安全的。

Objective To assess the effects of hyperbaric oxygen（HBO）on indolent prostate cancer on a murine model. Methods Human prostate cancer cell line PC-3 ceils were injected into 40 severe combined-irnmunodeficient mice. They were randomized to undergo 20 sessions of either HBO or normobaric air in standardized conditions, and observed for 4 weeks before the histological assessment of any palpable tumors developed. The analysis parameters included tumour volume, microvessel density, apoptosis markers （p53, p27） and proliferative index （Ki-67）. Results On the 28th day after tumor vaccination, the tumor volume was （425.8±13.9）mm^3 in HBO group and （433.6±12.8） mm^3 in normobaric air group （P〈0.05）. Microvessel density and Ki-67, p53, p27 protein expression were 69.7±9.5, （55. 2±6.7）%, （31. 2±5. 3）%, （80. 4±5.7）% in HBO group;77.1±8.7, （50.6 ± 7.3） %, （30.5±4.7） %, （85.3 ± 6.4） % in normobaric air group, respectively. There were no significant differences in both groups （P〉0.05）. Conclusions HBO does not accelerate the growth of indolent prostate cancer in murine model. This result suggests that HBO does not increase the risk of residual prostate cancer reactivation when it is used to manage radiation-induced hemorrhagic cystitis in patients treated by pelvic radiotherapy for prostate cancer.