两种接种密度骨髓基质干细胞复合β-TCP对兔腰椎横突间融合的效果

Spinal fusion of lumbar intertransverse process in rabbits using two different densities of autologous marrow mesenchymal stem cells combined with β-TCP

[目的]观察两种不同接种密度的骨髓基质干细胞(bone marrow mesenchymal stem cells,BMSCs),在成骨诱导分化后复合β-TCP应用于兔腰椎横突间脊柱融合的术后融合效果。[方法]应用BMSCs/β-TCP复合体对随机分为两组(低密度接种组和高密度接种组)的实验兔进行腰椎横突间非去皮质骨脊柱融合术,观察两组动物术后大体腰椎融合率、影像学特征、骨矿含量(bone mineralization content,BMC),骨密度(bone mineralization density,BMD)和矿化组织体积(bone mineralization tissue volume,BMV)及组织形态学变化。[结果]与低密度接种组比较,高密度接种组术后融合率明显提高,可达到71.4%(P<0.05)。显微CT图像结果显示高密度接种组横突间不仅新骨形成量多,而且融合稳固,且其BMC、BMD和BMV及新骨生成率均高于低密度接种组(P<0.05)。[结论]使用接种密度为10×106cells/ml的人工骨具有较好的融合效果,融合率可达到71.4%,这一点为临床应用BMSCs复合生物材料体外构建人工骨应用于非去皮质骨脊柱后路融合有一定的指导意义。

[ Objective ] To investigate the effects of two different densities of bone marrow mesenchymal stem cells （BMSCs） combined with β-TCP on spinal fusion in rabbits. [ Method ] Spinal fusion surgery of lumber intertransverse process were performed using MSCs/β-TCP as a graft in two groups of rabbits （low density group versus high density group） ,and rate of lumbar fusion,image characteristics, bone mineralization content, bone mineralization density, bone mineralization tissue volume and rate of new bone formation were observed. [ Result] Compared with the low intensity group, the rate of lumbar fusion was greatly improved in the high density group（ P 〈 0. 05 ）, and the values of BMC, BMD, and BMV, and the rate of new bone formation were also higher in high density group （P 〈 0.05 ）. [ Conclusion ] β-TCP combined with BMSCs of the density of 10 × 10^6 cells/ ml could enhance spinal fusion and this could guide the surgeons as to that one of the densities is more suitable in clinical practice.