HPLC-ELSD测定威灵仙中齐墩果酸的含量

Determination of Oleanolic Acid in Weilingxian by HPLC-ELSD

目的建立威灵仙中齐墩果酸的高效液相色谱-蒸发光散射法测定方法。方法采用SumFireTMC18(4.6mm×250mm,5μm)色谱柱,柱温40℃;乙腈-水(85∶15)为流动相,流速1.0mL·min-1;蒸发光散射检测器漂移管温度85℃,空气流速2.3L·min-1。结果齐墩果酸在0.214～1.92μg内线性关系良好,r=0.9999,平均回收率为98.8%,RSD为0.9%(n=6)。结论该方法简便,快速,准确,可作为威灵仙的质量控制。

OBJECTIVE To establish an HPLC-ELSD method for the determination of oleanolic acid in Weilingxian. METHODS Using SumFire^TM C18 column（4.6 mm×250 mm, 5 μm） at 40 ℃, acetonitrile -water（85 ： 15） was used as the moble phase, the flow rate was 1.0 mL·min^-1. An evaporative light-scattering detector（ELSD） was used as detector with gas flow rate of 2.3 L·min^-1 and drife tube temperature of 85 ℃. RESULTS The oleanolic acid had a good linearity relationship between 0.214-1.92 μg（r=-0.999 9）. The average recovery was 98.8% with RSD = 0.9%（n=6）. CONCLUSION The method is simple, rapid and accurate, suitable for the quality control of Weilingxian.